Combination of vitamin D and probiotics inhibits chemically induced colorectal carcinogenesis in Wistar rats

异常隐窝病灶 维生素 癌变 地穴 增殖细胞核抗原 免疫组织化学 结直肠癌 生物 内科学 癌症研究 医学 癌症 结肠疾病
作者
Carolina S. de Oliveira,Mariane Minussi Baptistella,Alexia P. Siqueira,Michele Oliveira Carvalho,Luiz Fernando Ramos,Bianca Silva Souto,Leonardo Augusto de Almeida,Elda Gonçalves dos Santos,Rômulo Dias Novaes,Ester S.C. Nogueira,Pollyanna Francielli de Oliveira
出处
期刊:Life Sciences [Elsevier]
卷期号:322: 121617-121617 被引量:3
标识
DOI:10.1016/j.lfs.2023.121617
摘要

The modulation of inflammatory elements, cell differentiation and proliferation by vitamin D and the role of probiotics in the intestinal microbiota and immunogenic response have sparked interest in the application of both in chemotherapeutics and chemoprevention of colorectal tumors.The present study aimed to investigate the effects of isolated and/or combined treatment of vitamin D3 and probiotics on colorectal carcinogenesis.Pre-neoplastic lesions were induced with 1,2-dimethylhydrazine in the colon of Wistar rats, which were treated with probiotics and/or vitamin D in three different approaches (simultaneous, pre-, and post-treatment). We investigated the frequency of aberrant crypt foci (ACF) and aberrant crypt (AC) in the distal colon, fecal microbiome composition, gene and protein expression through immunohistochemical and RT-PCR assays, and general toxicity through water consumption and weight gain monitoring.Results confirm the systemic safety of treatments, and show a protective effect of vitamin D and probiotics in all approaches studied, as well as in combined treatments, with predominance of different bacterial phyla compared to controls. Treated groups show different levels of Nrf2, GST, COX2, iNOS, β-catenin and PCNA expression.These experimental conditions explore the combination of vitamin D and probiotics supplementation at low doses over pathways involved in distinct stages of colorectal carcinogenesis, with results supporting its application in prevention and long-term strategies.
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