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Gut microbiota-derived LCA mediates the protective effect of PEDV infection in piglets

生物 罗伊乳杆菌 乳酸菌 微生物学 肠道菌群 细菌 免疫学 遗传学
作者
Jun-Hong Xing,Tianming Niu,Bo-Shi Zou,Guilian Yang,Chunwei Shi,Qingsong Yan,Mingjie Sun,Tong Yu,Shumin Zhang,Xi-Ze Feng,Shu-Hui Fan,Haibin Huang,Junhong Wang,Ming‐Han Li,Yanlong Jiang,Jianzhong Wang,Xin Cao,Nan Wang,Yan Zeng,Jingtao Hu,Di Zhang,Wu-Sheng Sun,Wentao Yang,Chunfeng Wang
出处
期刊:Microbiome [BioMed Central]
卷期号:12 (1) 被引量:3
标识
DOI:10.1186/s40168-023-01734-4
摘要

Abstract Background The gut microbiota is a critical factor in the regulation of host health, but the relationship between the differential resistance of hosts to pathogens and the interaction of gut microbes is not yet clear. Herein, we investigated the potential correlation between the gut microbiota of piglets and their disease resistance using single-cell transcriptomics, 16S amplicon sequencing, metagenomics, and untargeted metabolomics. Results Porcine epidemic diarrhea virus (PEDV) infection leads to significant changes in the gut microbiota of piglets. Notably, Landrace pigs lose their resistance quickly after being infected with PEDV, but transplanting the fecal microbiota of Min pigs to Landrace pigs alleviated the infection status. Macrogenomic and animal protection models identified Lactobacillus reuteri and Lactobacillus amylovorus in the gut microbiota as playing an anti-infective role. Moreover, metabolomic screening of the secondary bile acids’ deoxycholic acid (DCA) and lithocholic acid (LCA) correlated significantly with Lactobacillus reuteri and Lactobacillus amylovorus , but only LCA exerted a protective function in the animal model. In addition, LCA supplementation altered the distribution of intestinal T-cell populations and resulted in significantly enriched CD8 + CTLs, and in vivo and in vitro experiments showed that LCA increased SLA-I expression in porcine intestinal epithelial cells via FXR receptors, thereby recruiting CD8 + CTLs to exert antiviral effects. Conclusions Overall, our findings indicate that the diversity of gut microbiota influences the development of the disease, and manipulating Lactobacillus reuteri and Lactobacillus amylovorus , as well as LCA, represents a promising strategy to improve PEDV infection in piglets.
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