微流控
电流体力学
分类
按需
计算机硬件
电润湿
电压
吞吐量
信号(编程语言)
计算机科学
数字微流体
纳米技术
材料科学
工程类
化学
电气工程
电极
电信
多媒体
物理化学
无线
程序设计语言
作者
Anle Ge,Zhidian Diao,Yuandong Li,Chaojie Cui,Xixian Wang,Yang Liu,Jian Xu,Bo Ma
标识
DOI:10.1016/j.snb.2024.135334
摘要
Droplet microfluidics has revolutionized single-cell analysis, allowing for ultra-high-throughput sorting of droplets to meet the demands of single-cell sorting. However, accurately and efficiently dispensing target droplets into specific containers for downstream analysis has remained a challenge. Here, we present an integrated microfluidic droplet dispenser based on the electrohydrodynamic (EHD) principle, capable of on-demand single droplet dispensing with high accuracy. Our system uses optical signal activation and EHD actuation to isolate and dispense droplets. The microfluidic droplet auto dispenser consists of four main modules: a pump-drive chip module, an optical automatic signal recognition module, a high voltage control module, and an automated X-Y translation stage module. By optimizing the chip structure and voltage parameter, this platform successfully isolates single Escherichia coli (E. coli) cells onto a Petri dish for downstream cultivation and heterogeneity analysis. In addition, the dispenser microfluidic system dispenses the sorted droplets in a "one droplet, one well" manner, providing an automated method for droplet interface from the micro- to macro- platform. This dispenser system provides a platform for phenotypic screening of single colony pick and single cell analysis
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