3D生物打印
生物人工肝装置
移植
肝移植
肝衰竭
肝功能
医学
组织工程
肝细胞
生物医学工程
生物
外科
体外
内科学
生物化学
作者
Bo Deng,Yue Ma,Jialyu Huang,Rong He,Miaomiao Luo,Lina Mao,Enhua Zhang,Yuanyuan Zhao,Xiaoli Wang,Qiang‐Song Wang,Mingchang Pang,Yilei Mao,Huayu Yang,Lanxia Liu,Pengyu Huang
出处
期刊:Science Advances
[American Association for the Advancement of Science (AAAS)]
日期:2024-06-07
卷期号:10 (23)
标识
DOI:10.1126/sciadv.ado1550
摘要
The utilization of three-dimensional (3D) bioprinting technology to create a transplantable bioartificial liver emerges as a promising remedy for the scarcity of liver donors. This study outlines our strategy for constructing a 3D-bioprinted liver, using in vitro–expanded primary hepatocytes recognized for their safety and enhanced functional robustness as hepatic cell sources for bioartificial liver construction. In addition, we have developed bioink biomaterials with mechanical and rheological properties, as well as printing capabilities, tailored for 3D bioprinting. Upon heterotopic transplantation into the mesentery of tyrosinemia or 90% hepatectomy mice, our 3D-bioprinted liver effectively restored lost liver functions, consequently extending the life span of mice afflicted with liver injuries. Notably, the inclusion of an artificial blood vessel in our 3D-bioprinted liver allowed for biomolecule exchange with host blood vessels, demonstrating, in principle, the rapid integration of the bioartificial liver into the host vascular system. This model underscores the therapeutic potential of transplantation for the treatment of liver failure diseases.
科研通智能强力驱动
Strongly Powered by AbleSci AI