In response to bacteria, neutrophils release extracellular vesicles capable of initiating thrombin generation through DNA-dependent and independent pathways

生物 细胞外小泡 凝血酶生成 中性粒细胞胞外陷阱 微生物学 细菌 凝血酶 DNA 细胞外 细胞生物学 生物化学 免疫学 遗传学 炎症 血小板
作者
Kaitlyn M. Whitefoot-Keliin,Chase C Benaske,Edwina R. Allen,Mariana T Guerrero,Justin W Grapentine,Benjamin Schiff,Andrew R. Mahon,Mallary C. Greenlee‐Wacker
出处
期刊:Journal of Leukocyte Biology [Oxford University Press]
卷期号:116 (6): 1223-1236 被引量:5
标识
DOI:10.1093/jleuko/qiae125
摘要

Abstract Neutrophils release extracellular vesicles, and some subsets of neutrophil-derived extracellular vesicles are procoagulant. In response to Staphylococcus aureus, neutrophils produce extracellular vesicles that associate electrostatically with neutrophil extracellular traps. DNA in neutrophil extracellular traps is procoagulant, but whether neutrophil extracellular vesicles produced during bacterial challenge have similar activity is unknown. Given that extracellular vesicle activity is agonist and cell-type dependent and coagulation contributes to sepsis, we hypothesized that sepsis-causing bacteria increase production of neutrophil-derived extracellular vesicles, as well as extracellular vesicle–associated DNA, and intact extracellular vesicles and DNA cause coagulation. We recovered extracellular vesicles from neutrophils challenged with S. aureus, Staphylococcus epidermidis, Escherichia coli, and Pseudomonas aeruginosa and measured associated DNA and procoagulant activity. Extracellular vesicles from S. aureus–challenged neutrophils, which were previously characterized, displayed dose-dependent procoagulant activity as measured by thrombin generation in platelet-poor plasma. Extracellular vesicle lysis and DNase treatment reduced thrombin generation by 90% and 37%, respectively. S. epidermidis, E. coli, and P. aeruginosa also increased extracellular vesicle production and extracellular vesicle–associated extracellular DNA, and these extracellular vesicles were also procoagulant. Compared to spontaneously released extracellular vesicles, which demonstrated some ability to amplify factor XII–dependent coagulation in the presence of an activator, only extracellular vesicles produced in response to bacteria could initiate the pathway. S. aureus and S. epidermidis extracellular vesicles had more surface-associated DNA than E. coli and P. aeruginosa extracellular vesicles, and S. aureus and S. epidermidis extracellular vesicles contributed to initiation and amplification of thrombin generation in a DNA-dependent manner. However, DNA on E. coli or P. aeruginosa extracellular vesicles played no role, suggesting that neutrophils release procoagulant extracellular vesicles, which can activate the coagulation cascade through both DNA-dependent and independent mechanisms.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
酷波er应助xxxxx采纳,获得10
刚刚
刚刚
BINGBING1230发布了新的文献求助100
1秒前
朱朱朱完成签到,获得积分10
1秒前
科目三应助笨笨的仙人掌采纳,获得10
1秒前
乐乐应助cute666采纳,获得10
1秒前
王怡发布了新的文献求助10
2秒前
gqp完成签到,获得积分10
2秒前
浮浮世世发布了新的文献求助10
2秒前
2秒前
赵念婉完成签到,获得积分10
3秒前
4秒前
英吉利25发布了新的文献求助10
5秒前
星辰大海应助WB采纳,获得10
5秒前
花开富贵完成签到,获得积分10
5秒前
5秒前
5秒前
852应助凉雨街采纳,获得10
6秒前
樱桃味的火苗完成签到,获得积分10
6秒前
许晴发布了新的文献求助10
6秒前
8R60d8应助爱笑的雪糕采纳,获得10
7秒前
7秒前
7秒前
所所应助bixun采纳,获得10
7秒前
李爱国应助ppapp采纳,获得10
7秒前
飞鸿影下完成签到 ,获得积分10
8秒前
勤恳的一斩完成签到,获得积分10
8秒前
豆豆完成签到,获得积分10
8秒前
8秒前
dongdongqiang完成签到,获得积分10
9秒前
鹤雪完成签到,获得积分20
9秒前
阿北完成签到,获得积分10
9秒前
10秒前
简柠完成签到,获得积分10
10秒前
10秒前
10秒前
情怀应助单身的世倌采纳,获得10
10秒前
10秒前
咕噜咕噜完成签到 ,获得积分10
10秒前
高分求助中
(应助此贴封号)【重要!!请各用户(尤其是新用户)详细阅读】【科研通的精品贴汇总】 10000
网络安全 SEMI 标准 ( SEMI E187, SEMI E188 and SEMI E191.) 1000
计划经济时代的工厂管理与工人状况(1949-1966)——以郑州市国营工厂为例 500
INQUIRY-BASED PEDAGOGY TO SUPPORT STEM LEARNING AND 21ST CENTURY SKILLS: PREPARING NEW TEACHERS TO IMPLEMENT PROJECT AND PROBLEM-BASED LEARNING 500
Why America Can't Retrench (And How it Might) 400
Two New β-Class Milbemycins from Streptomyces bingchenggensis: Fermentation, Isolation, Structure Elucidation and Biological Properties 300
Modern Britain, 1750 to the Present (第2版) 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 物理化学 基因 催化作用 遗传学 冶金 电极 光电子学
热门帖子
关注 科研通微信公众号,转发送积分 4614581
求助须知:如何正确求助?哪些是违规求助? 4018748
关于积分的说明 12439646
捐赠科研通 3701503
什么是DOI,文献DOI怎么找? 2041241
邀请新用户注册赠送积分活动 1073983
科研通“疑难数据库(出版商)”最低求助积分说明 957639