Abstract 14574: Cellular Population Evolution and Differential Gene Expression Patterns of Human Aorta in Different Life Stages Revealed by Single-Cell Transcriptome Analysis

间充质干细胞 细胞 人口 成纤维细胞 细胞外基质 转录组 生物 病理 细胞生物学 基因表达 医学 电池类型 免疫学 分子生物学 细胞培养 基因 遗传学 环境卫生
作者
Bin Zhang,Ruicong Guan,Kuan Zeng,Huiqi Jiang,Minnan Gao,Lu Zhang,Liu CongYong,Jianfen Li,Jingwen Li,Han Jingjing,Baoping Deng,David YB Deng,Qing Zhang,Mo Yang,Yongqiang Yang
出处
期刊:Circulation [Ovid Technologies (Wolters Kluwer)]
卷期号:146 (Suppl_1)
标识
DOI:10.1161/circ.146.suppl_1.14574
摘要

Introduction: Cellular characteristics and gene expression patterns of human aortic tissue is biological basis for understanding pathogenesis of aortic diseases, and still unclear. Our aim was to investigate cellular population evolution and differential gene expression patterns of human aorta in different life stages. Methods: Single-cell transcriptome sequencing was applied to study differential gene expression pattern and cellular population of entire ascending aortic walls from six healthy donors: 4 in younger group (3 months, 1 year, 3 and 12 years of age) and 2 in aged group (57 and 58 years of age), 50% males. Pseudotime trajectory and cell-to-cell communication analysis of the annotated cell subclusters were preformed to analyze development and evolution of cells. Histological examination of the specimens was carried out by using routine and immunohistochemical stains to validate findings of single-cell sequencing. Results: Ten main cell types (27 high-resolution subtypes, totally 65,470 cells) were identified in the specimens, including endothelial cell, smooth muscle cell, fibroblast, mesenchymal cell, mesenchymal-fibroblast cell, macrophage, and T cell. As donors age increased, proportion of non-immune cells such as endothelial cell decreased from 22.47% to 10.37%, smooth muscle cell from 20.40% to 18.50%, fibroblasts from 30.21% to 13.29%, and mesenchymal cell from 5.12% to 1.55%; while proportion of immune cells such as macrophages increased from 10.94 % to 17.02 % ; and T cells from 3.80% to 26.01%. Synthetic smooth muscle cell, fibroblasts of extracellular matrix synthesis, and mesenchymal cells expressing osteocalcin/matrix Gla family genes (MAG, logFC: 4.0, P<0.01) were significantly increased in the aged donors as well as the ligand-receptor pairs between macrophages and T cells mainly in endocrine resistance related pathway. M1 (CD86) and M2 (CD163) macrophage signature antibodies were slightly elevated in the aged donors which correlated well to the findings from single-cell sequencing. Conclusions: Cellular population and differential gene expression patterns in human aortic tissue are under dynamic evolution in the life circle. Immune cells infiltration increases while non-immune cells decrease as age increases.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
黄花发布了新的文献求助10
刚刚
1秒前
2秒前
娜娜发布了新的文献求助10
3秒前
4秒前
5秒前
5秒前
6秒前
7秒前
一粒星辰完成签到,获得积分10
8秒前
8秒前
Bake完成签到,获得积分10
9秒前
苟发东发布了新的文献求助10
9秒前
9秒前
9秒前
泥泞o完成签到,获得积分10
10秒前
hennie发布了新的文献求助10
10秒前
李健应助呜呼啦呼采纳,获得10
11秒前
11秒前
科目三应助fd采纳,获得10
11秒前
刁德一发布了新的文献求助10
11秒前
11秒前
橙橙橙发布了新的文献求助10
12秒前
丸子歪歪发布了新的文献求助30
12秒前
Liang发布了新的文献求助10
13秒前
p_kunnnn发布了新的文献求助10
13秒前
13秒前
gnufgg完成签到,获得积分10
14秒前
14秒前
15秒前
15秒前
桐桐应助王小妖采纳,获得10
15秒前
秋裤掉了发布了新的文献求助10
15秒前
去码头整点薯条完成签到,获得积分10
16秒前
17秒前
17秒前
昭明完成签到,获得积分10
17秒前
18秒前
大胆的夏天完成签到,获得积分10
19秒前
虞不斜完成签到 ,获得积分10
19秒前
高分求助中
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Very-high-order BVD Schemes Using β-variable THINC Method 870
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3256085
求助须知:如何正确求助?哪些是违规求助? 2898220
关于积分的说明 8300473
捐赠科研通 2567352
什么是DOI,文献DOI怎么找? 1394499
科研通“疑难数据库(出版商)”最低求助积分说明 652817
邀请新用户注册赠送积分活动 630511