斑马鱼
染色
视网膜
组织学
生物
解剖
胚胎
形态学(生物学)
视杯(胚胎学)
细胞生物学
表型
眼睛发育
遗传学
神经科学
基因
作者
Yujin Lee,Jae Wook Yang
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 191-198
标识
DOI:10.1007/978-1-0716-3255-0_15
摘要
Histology is a technique used to study the morphology of cell structures by cutting samples into thin sections. Histological cross-section and staining are the techniques needed to visualize the morphology of cell tissues. A suitable tissue staining experiment was created to observe changes in the retinal layer in zebrafish embryos. Zebrafish have a human-like visual system, retina, and eye structures. Due to the small size of zebrafish and undeveloped bones in the embryonic stage, the resistance through cross-section is inevitably small. Here, we present optimized protocol changes in eye tissue of zebrafish using frozen blocks.
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