Distinct bacterial population dynamics and disease dissemination after biofilm dispersal and disassembly

生物膜 生物扩散 生物 细菌 微生物学 殖民地化 人口 寄主(生物学) 铜绿假单胞菌 生态学 遗传学 社会学 人口学
作者
Yeping Ma,Yanlin Deng,Haojun Hua,Bee Luan Khoo,Song Lin Chua
出处
期刊:The ISME Journal [Springer Nature]
卷期号:17 (8): 1290-1302 被引量:5
标识
DOI:10.1038/s41396-023-01446-5
摘要

Abstract Microbial communities that form surface-attached biofilms must release and disperse their constituent cells into the environment to colonize fresh sites for continued survival of their species. For pathogens, biofilm dispersal is crucial for microbial transmission from environmental reservoirs to hosts, cross-host transmission, and dissemination of infections across tissues within the host. However, research on biofilm dispersal and its consequences in colonization of fresh sites remain poorly understood. Bacterial cells can depart from biofilms via stimuli-induced dispersal or disassembly due to direct degradation of the biofilm matrix, but the complex heterogeneity of bacterial populations released from biofilms rendered their study difficult. Using a novel 3D-bacterial “biofilm-dispersal-then-recolonization” (BDR) microfluidic model, we demonstrated that Pseudomonas aeruginosa biofilms undergo distinct spatiotemporal dynamics during chemical-induced dispersal (CID) and enzymatic disassembly (EDA), with contrasting consequences in recolonization and disease dissemination. Active CID required bacteria to employ bdlA dispersal gene and flagella to depart from biofilms as single cells at consistent velocities but could not recolonize fresh surfaces. This prevented the disseminated bacteria cells from infecting lung spheroids and Caenorhabditis elegans in on-chip coculture experiments. In contrast, EDA by degradation of a major biofilm exopolysaccharide (Psl) released immotile aggregates at high initial velocities, enabling the bacteria to recolonize fresh surfaces and cause infections in the hosts efficiently. Hence, biofilm dispersal is more complex than previously thought, where bacterial populations adopting distinct behavior after biofilm departure may be the key to survival of bacterial species and dissemination of diseases.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
打打应助谦让的青亦采纳,获得10
刚刚
1秒前
1秒前
1秒前
1秒前
干净傲霜发布了新的文献求助10
2秒前
CodeCraft应助温暖的胳肢窝采纳,获得10
2秒前
Ava应助请叫我湿人人采纳,获得10
3秒前
5秒前
心灵美复天完成签到,获得积分10
6秒前
沉默的婴发布了新的文献求助10
7秒前
xkk发布了新的文献求助10
9秒前
9秒前
李爱国应助独特元蝶采纳,获得10
11秒前
13秒前
畅快沁完成签到,获得积分10
13秒前
沿海地带应助18166992885采纳,获得20
13秒前
14秒前
15秒前
不懈奋进应助dablack采纳,获得30
15秒前
小杰完成签到,获得积分10
17秒前
19秒前
19秒前
21秒前
凝韵发布了新的文献求助20
24秒前
25秒前
诚心的奎发布了新的文献求助10
25秒前
25秒前
25秒前
26秒前
Akim应助枯木逢生i采纳,获得10
26秒前
cocolu应助甜甜秋荷采纳,获得30
27秒前
行路难完成签到,获得积分10
28秒前
Yuting发布了新的文献求助10
28秒前
独特元蝶发布了新的文献求助10
28秒前
善学以致用应助快乐小子采纳,获得10
29秒前
mengdewen发布了新的文献求助10
29秒前
傻子与白痴完成签到,获得积分10
30秒前
sharky发布了新的文献求助50
30秒前
30秒前
高分求助中
Solution Manual for Strategic Compensation A Human Resource Management Approach 1200
Natural History of Mantodea 螳螂的自然史 1000
Glucuronolactone Market Outlook Report: Industry Size, Competition, Trends and Growth Opportunities by Region, YoY Forecasts from 2024 to 2031 800
A Photographic Guide to Mantis of China 常见螳螂野外识别手册 800
Zeitschrift für Orient-Archäologie 500
Smith-Purcell Radiation 500
Autoregulatory progressive resistance exercise: linear versus a velocity-based flexible model 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 细胞生物学 免疫学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3343504
求助须知:如何正确求助?哪些是违规求助? 2970547
关于积分的说明 8644499
捐赠科研通 2650612
什么是DOI,文献DOI怎么找? 1451426
科研通“疑难数据库(出版商)”最低求助积分说明 672137
邀请新用户注册赠送积分活动 661545