化学
阿布茨
DPPH
多糖
岩藻糖
葡萄糖醛酸
鼠李糖
半乳糖
甘露糖
木糖
生物
抗氧化剂
核化学
色谱法
微生物学
食品科学
有机化学
生物化学
发酵
作者
Hongjie Yuan,Lin Dong,Zhiyuan Zhang,Yan He,Xia Ma
标识
DOI:10.1016/j.fshw.2022.03.030
摘要
Tremella fuciformis polysaccharide (TFP) is an important bioactive substance in T. fuciformis , which contributes to its application as medicine and food. In the present work, TFP was extracted with enzymatic combined with wet beating and its physicochemical as well as biological activities were investigated in the study. The result showed that the purity of TFP1 reached 92.14% ( m / m ). The TFP1, purified by DEAE column, had a molecular weight ( M w ) of 5.8 × 10 3 kDa and consisted mainly of mannose, xylose, fucose, glucose and glucuronic acid at a molar ratio of 1.91: 0.1: 2.49: 6.23: 0.95. The structure of TFP1 was preliminarily investigated by infrared spectroscopy, nuclear magnetic resonance and atomic force microscope (AFM) analysis. Moreover, the antioxidant assays showed that TFP1 could scavenge DPPH, ABTS + and hydroxyl radicals. The excellent water holding capacity of TFP1 implied its application in the food, pharmaceutical andcosmetic industries.
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