Photoelectrochemical Sensors with Near-Infrared-Responsive Reduced Graphene Oxide and MoS2 for Quantification of Escherichia Coli O157:H7

The photoelectric response is crucial for photocatalysis, having applications in solar cells and photoelectrochemical (PEC) sensors. In this study, we demonstrate improvements in the near-infrared (NIR)-light-driven PEC response via synergism between reduced graphene oxide (rGO) and MoS₂. Intriguingly, rGO modulates the morphology of MoS₂, facilitating carrier generation and migration, improving the PEC performance of the resultant rGO-MoS₂ sheets (GMS), and yielding an approximately 8-fold increase in the photocurrent compared to that of the pure MoS₂. Based on these findings, a NIR-responsive PEC immunosensing platform for the "turn-on" analysis of Escherichia coli O157:H7 on 980 nm light irradiation is reported. Specifically, the device is a three-dimensional magnetic screen-printed paper-based electrode assembled on a home-made PEC cell, and it enables integrated separation and detection. Using a sandwich-type immunocomplex bridged by E. coli O157:H7 and a GMS PEC probe, the immunosensing platform detected E. coli O157:H7 between 5.0 and 5.0 × 10⁶ CFU mL^-1, having an extremely low detection limit of 2.0 CFU mL^-1. Further, the assay enables the direct analysis of E. coli O157:H7 in milk without the need for pretreatment. Our findings suggest directions for the development of NIR-responsive paper-based PEC materials for portable biomolecule sensing.