Construction and validation of a mitochondria-associated genes prognostic signature and immune microenvironment characteristic of sepsis

败血症 免疫系统 列线图 比例危险模型 单变量分析 免疫学 生物 内科学 医学 多元分析
作者
Qi Shu,Yuanlin Du,Han She,Jiaping Mo,Zhenjie Zhu,Like Zhong,Fugen He,Jingsheng Fan,Junfeng Zhu
出处
期刊:International Immunopharmacology [Elsevier BV]
卷期号:126: 111275-111275 被引量:8
标识
DOI:10.1016/j.intimp.2023.111275
摘要

Sepsis is a common critical condition seen in clinical settings, with mitochondrial dysfunction playing an important role in the progression of sepsis. However, a mitochondrial prognosis model related to sepsis has not been established yet, and the relationship between the sepsis immune microenvironment and mitochondria remains unclear. Sepsis prognostic mitochondria-associated genes (MiAGs) were screened by univariate Cox, multivariate Cox, and LASSO analysis from the GEO dataset. Consensus Cluster was used to analyze MiAGs-based molecular subtypes for sepsis. The ESTIMATE and ssGSEA algorithms were used to analyze the situation of sepsis immune cell infiltration and its relation to MiAGs. Further, MiAGs score was calculated to construct a sepsis prognosis risk model to predict the prognosis of sepsis patients. Clinical blood samples were used to investigate the expression level of selected MiAGs in sepsis. Single-cell sequencing analysis, mitochondrial membrane potential (MMP), reactive oxygen species (ROS), and ATP detection were used to verify the influence of MiAGs on mitochondrial dysfunction in sepsis. A total of 5 MiAGs of sepsis were screened. Based on MiAGs, sepsis MiAGs subtypes were analyzed, where Cluster A had a better prognosis than Cluster B, and there were significant differences in immune infiltration between the two clusters. The sepsis mitochondrial prognosis model suggested that the high MiAG score group had a shorter survival time compared to the low MiAG score group. Significant differences were also observed in the immune microenvironment between the high and low MiAG score groups. Prognostic analysis and the Nomogram indicated that the MiAG score is an independent prognostic factor in sepsis. Single-cell sequencing analysis exhibited the possible influence of MiAGs on the mitochondrial function of monocytes. Finally, the downregulation of the COX7B could effectively improve mitochondrial function in the LPS-stimulated sepsis model. Our findings suggest that MiAGs can be used to predict the prognosis of sepsis and that regulating the mitochondrial prognostic gene COX7B can effectively improve the mitochondrial function of immune cells in sepsis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
桐桐应助Bailey采纳,获得10
刚刚
elerain完成签到,获得积分10
2秒前
黎乐荷发布了新的文献求助10
2秒前
5秒前
Owen应助puzi采纳,获得10
5秒前
8秒前
Dr.Liujun发布了新的文献求助10
11秒前
adi发布了新的文献求助10
12秒前
12秒前
12秒前
sys完成签到,获得积分10
12秒前
Mandy完成签到,获得积分10
13秒前
14秒前
15秒前
ZWGS发布了新的文献求助30
16秒前
脑洞疼应助cindywu采纳,获得10
18秒前
18秒前
NexusExplorer应助Skuld采纳,获得10
19秒前
Dr.Liujun完成签到,获得积分10
19秒前
ixueyi发布了新的文献求助10
19秒前
20秒前
22秒前
木木应助qiqi采纳,获得10
22秒前
22秒前
girl发布了新的文献求助10
23秒前
27秒前
xuxu发布了新的文献求助10
27秒前
丘比特应助kaka采纳,获得10
29秒前
jszhoucl发布了新的文献求助10
30秒前
大头完成签到,获得积分10
32秒前
32秒前
奥特曼关注了科研通微信公众号
34秒前
YYJ发布了新的文献求助10
34秒前
Ren应助girl采纳,获得10
34秒前
留胡子的紫槐完成签到,获得积分10
36秒前
顾矜应助好想走到伯纳乌采纳,获得10
37秒前
38秒前
Skuld发布了新的文献求助10
39秒前
欧维完成签到 ,获得积分10
41秒前
41秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3988975
求助须知:如何正确求助?哪些是违规求助? 3531316
关于积分的说明 11253424
捐赠科研通 3269917
什么是DOI,文献DOI怎么找? 1804830
邀请新用户注册赠送积分活动 882063
科研通“疑难数据库(出版商)”最低求助积分说明 809068