微载波
胚状体
诱导多能干细胞
细胞培养
细胞生物学
细胞
悬浮培养
干细胞
化学
生物
胚胎干细胞
生物技术
生物化学
遗传学
基因
作者
Yasuhiko Tabata,Joanna Idaszek,Akon Higuchi
出处
期刊:Progress in Molecular Biology and Translational Science
日期:2023-01-01
卷期号:: 109-127
标识
DOI:10.1016/bs.pmbts.2023.04.009
摘要
Conventional two-dimensional (2-D) cultivation are easy to utilize for human pluripotent stem (hPS) cell cultivation in standard techniques and are important for analysis or development of the signal pathways to keep pluripotent state of hPS cells cultivated on 2-D cell culture materials. However, the most efficient protocol to prepare hPS cells is the cell culture in a three dimensional (3-D) cultivation unit because huge numbers of hPS cells should be utilized in clinical treatment. Some 3-D cultivation strategies for hPS cells are considered: (a) microencapsulated cell cultivation in suspended hydrogels, (b) cell cultivation on microcarriers (MCs), (c) cell cultivation on self-aggregated spheroid [cell aggregates; embryoid bodies (EBs) and organoids], (d) cell cultivation on microfibers or nanofibers, and (e) cell cultivation in macroporous scaffolds. These cultivation ways are described in this chapter.
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