列线图
免疫系统
腺癌
医学
免疫疗法
基因敲除
基因
癌症研究
肿瘤科
肺癌
内科学
生物
免疫学
癌症
遗传学
作者
Qianhe Ren,Qifan Li,Chenye Shao,Pengpeng Zhang,Zhuangzhuang Hu,Jun Li,Wei Wang,Yue Yu
出处
期刊:BMC Cancer
[Springer Nature]
日期:2023-09-23
卷期号:23 (1)
被引量:3
标识
DOI:10.1186/s12885-023-11249-8
摘要
Abstract Background Lung adenocarcinoma (LUAD) is an extraordinarily malignant tumor, with rapidly increasing morbidity and poor prognosis. Immunotherapy has emerged as a hopeful therapeutic modality for lung adenocarcinoma. Furthermore, a prognostic model (based on immune genes) can fulfill the purpose of early diagnosis and accurate prognostic prediction. Methods Immune-related mRNAs (IRmRNAs) were utilized to construct a prognostic model that sorted patients into high- and low-risk groups. Then, the prediction efficacy of our model was evaluated using a nomogram. The differences in overall survival (OS), the tumor mutation landscape, and the tumor microenvironment were further explored between different risk groups. In addition, the immune genes comprising the prognostic model were subjected to single-cell RNA sequencing to investigate the expression of these immune genes in different cells. Finally, the functions of BIRC5 were validated through in vitro experiments. Results Patients in different risk groups exhibited sharply significant variations in OS, pathway activity, immune cell infiltration, mutation patterns, and immune response. Single-cell RNA sequencing revealed that the expression level of BIRC5 was significantly high in T cells. Cell experiments further revealed that BIRC5 knockdown markedly reduced LUAD cell proliferation. Conclusion This model can function as an instrumental variable in the prognostic, molecular, and therapeutic prediction of LUAD, shedding new light on the optimal clinical practice guidelines for LUAD patients.
科研通智能强力驱动
Strongly Powered by AbleSci AI