微小隐孢子虫
细胞凋亡
福克斯M1
下调和上调
小RNA
生物
细胞生长
细胞
癌症研究
自噬
微生物学
细胞周期
基因
生物化学
遗传学
作者
Qian Yao,Yingying Fan,Shuang Huang,Gui-Rong Hu,Jun-Ke Song,Xuefei Yang,Guang‐Hui Zhao
出处
期刊:Acta Tropica
[Elsevier]
日期:2024-01-01
卷期号:249: 107057-107057
标识
DOI:10.1016/j.actatropica.2023.107057
摘要
Cryptosporidium parvum could regulate the expression of microRNAs of epithelial cells to facilitate its intracellular propagation. MiR-4521 has been reported to play an important role during the development and progression of tumors and infectious diseases by regulating cell proliferation, apoptosis, and autophagy. However, the implication of miR-4521 during C. parvum infection was still unknown. In this study, the expression of miR-4521 was found to be upregulated in HCT-8 cells infected with C. parvum from 8 h post-infection (pi) to 48 hpi, and its upregulation would be related with the TLR4/NF-κB signal pathway during C. parvum infection. One potential target of miR-4521, foxm1, was down-regulated in HCT-8 cells from 24 hpi to 48 hpi, and the expression of foxm1 was negatively regulated by miR-4521. The target relationship between miR-4521 and foxm1 was further validated by using dual luciferase reporter assay. Further studies showed that miR-4521 promoted the propagation of C. parvum in HCT-8 cells through targeting foxm1 by regulating BCL2-mediating cell apoptosis. These results contribute to further understanding of the regulatory mechanisms of host miRNAs during Cryptosporidium infection.
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