Highly Dispersed 3C Silicon Carbide Nanoparticles with a Polydopamine/Polyglycerol Shell for Versatile Functionalization

Silicon carbide (SiC) nanoparticles containing lattice defects are attracting considerable attention as next-generation imaging probes and quantum sensors for visualizing and sensing life activities. However, SiC nanoparticles are not currently used in biomedical applications because of the lack of technology for controlling their physicochemical properties. Therefore, in this study, SiC nanoparticles are deaggregated, surface-coated, functionalized, and selectively labeled to biomolecules of interest. A thermal-oxidation chemical-etching method is developed for deaggregating and producing a high yield of dispersed metal-contaminant-free SiC nanoparticles. We further demonstrated a polydopamine coating with controllable thickness that can be used as a platform for decorating gold nanoparticles on the surface, enabling photothermal application. We also demonstrated a polyglycerol coating, which gives excellent dispersity to SiC nanoparticles. Furthermore, a single-pot method is developed to produce mono/multifunctional polyglycerol-modified SiC nanoparticles. Using this method, CD44 proteins on cell surfaces are selectively labeled through biotin-mediated immunostaining. The methods developed in this study are fundamental for applying SiC nanoparticles to biomedical applications and should considerably accelerate the development of various SiC nanoparticles to exploit their potential applications in bioimaging and biosensing.