金丝桃素
光敏剂
脂质体
聚乙烯亚胺
生物物理学
转染
材料科学
动态光散射
脂质双层
细胞毒性
光化学
化学
体外
纳米颗粒
膜
生物化学
纳米技术
生物
基因
药理学
作者
Hirva Shah,Samuel F. Schluter,Muhammad Umair Amin,Alice Abu Dayyih,Konrad Engelhardt,Shashank Reddy Pinnapireddy,Eduard Preis,Udo Bakowsky
标识
DOI:10.1021/acsami.4c10438
摘要
The lipopolyplex, a multicomponent nonviral gene carrier, generally demonstrates superior colloidal stability, reduced cytotoxicity, and high transfection efficiency. In this study, a new concept, photochemical reaction-induced transfection, using photosensitizer (PS)-loaded lipopolyplexes was applied, which led to enhanced transfection and cytotoxic effects by photoexcitation of the photosensitizer. Hypericin, a hydrophobic photosensitizer, was encapsulated in the lipid bilayer of liposomes. The preformed nanosized hypericin liposomes enclosed the linear polyethylenimine (lPEI)/pDNA polyplexes, resulting in the formation of hypericin lipopolyplexes (Hy-LPP). The diameters of Hy-LPP containing 50 nM hypericin and 0.25 μg of pDNA were 185.6 ± 7.74 nm and 230.2 ± 4.60 nm, respectively, measured by dynamic light scattering (DLS) and atomic force microscopy (AFM). Gel electrophoresis confirmed the encapsulation of hypericin and pDNA in lipopolyplexes. Furthermore, in vitro irradiation of intracellular Hy-LPP at radiant exposures of 200, 600, and 1000 mJ/cm
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