In-Cell Design of Solid Biocatalyst via CipA and Ferritin Fusion

Abstract Crystalline inclusion protein A (CipA) is a protein crystal that spontaneously forms in living cells. To construct a hybrid solid material, we engineered CipA by fusion with functionalized ferritin at the C-terminal. When ferritin was mutated with His residues, the resulting solid material exhibited peroxidase mimetic oxidation of 3, 3’, 5, 5’-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide. This study provides a simple method to develop functional solid materials.