Intestinal enteroendocrine cell subtype differentiation and hormone production in zebrafish

斑马鱼 肠内分泌细胞 激素 生物 细胞生物学 生产(经济) 内分泌系统 内科学 计算生物学 内分泌学 遗传学 医学 基因 经济 宏观经济学
作者
Margaret Morash,Richard G. Kay,Erik J. Soderblom,G. L. Maclean,Jia Wen,P Moore,Colin R. Lickwar,Fiona M. Gribble,Frank Reimann,Rodger A. Liddle,John F. Rawls
标识
DOI:10.1101/2025.01.17.633579
摘要

Enteroendocrine cells (EECs) are rare sensory cells in the intestinal epithelium that coordinate digestive physiology by secreting a diverse repertoire of peptide hormones. These hormones are the main effectors of EEC function, and their characterization requires direct observation by mass spectrometry due to the specialized protein cleavage and posttranslational modifications that yield their mature forms. Based on the distinct subset of hormones they predominantly secrete, EECs can be categorized into subtypes. How each EEC subtype is specified, however, remains poorly understood. Here we describe EEC subtype differentiation and hormone production in the zebrafish. Using single-cell RNA sequencing data, we identified EEC progenitors and six EEC subtypes in zebrafish and revealed that their expression profiles are consistent across larval and adult stages. Mass spectrometry analysis of isolated zebrafish EECs identified highly processed peptides derived from 18 of 21 hormone coding genes expressed by EECs, yielding a catalog of >400 unique EEC hormone peptides. We assembled reporters for zebrafish EEC subtypes to test the lineage relationships between EEC subtypes and the EEC progenitor population, which expresses neurogenin3. Despite its essential role in mammalian EEC differentiation, we found that selective cytotoxic ablation of neurogenin3+ cells in zebrafish only reduced a subset of EEC subtypes. Finally, we discovered that selective ablation of ghrelin+ EECs reduced a different subset of EEC subtypes, together suggesting that neurogenin3+ and ghrelin+ cells serve as distinct precursors for separate EEC subtypes. We anticipate these observations and resources will facilitate future studies in the zebrafish to discern the developmental biology, physiology, and endocrinology of EEC subtypes.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
hhhblabla应助yyyyyyy111采纳,获得10
3秒前
哈哈发布了新的文献求助10
4秒前
9秒前
11秒前
背后初南完成签到,获得积分10
12秒前
神勇馒头完成签到,获得积分10
12秒前
GGBOND发布了新的文献求助10
13秒前
13秒前
14秒前
以戈完成签到,获得积分10
16秒前
18秒前
泡泡脑瓜发布了新的文献求助10
19秒前
358489228完成签到,获得积分10
19秒前
20秒前
xww发布了新的文献求助10
22秒前
23秒前
神勇馒头发布了新的文献求助10
26秒前
26秒前
chen完成签到,获得积分10
28秒前
cindywu发布了新的文献求助10
28秒前
贰叁发布了新的文献求助10
29秒前
30秒前
量子星尘发布了新的文献求助10
31秒前
32秒前
Lu发布了新的文献求助10
34秒前
35秒前
泡泡脑瓜关注了科研通微信公众号
36秒前
丫丫丫完成签到,获得积分20
37秒前
无花果应助GGBOND采纳,获得10
37秒前
跳跃盼波完成签到,获得积分10
39秒前
喜悦松完成签到,获得积分10
40秒前
666完成签到,获得积分10
40秒前
Jessica完成签到,获得积分10
43秒前
43秒前
汉堡包应助666采纳,获得10
44秒前
Hello应助贰叁采纳,获得10
44秒前
上官若男应助hugdoggy采纳,获得10
46秒前
emmm完成签到,获得积分10
46秒前
斯文败类应助anna采纳,获得10
47秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989115
求助须知:如何正确求助?哪些是违规求助? 3531367
关于积分的说明 11253688
捐赠科研通 3269986
什么是DOI,文献DOI怎么找? 1804868
邀请新用户注册赠送积分活动 882078
科研通“疑难数据库(出版商)”最低求助积分说明 809105