Receptor-like cytoplasmic kinases belonging to different subfamilies mediate immune responses downstream of the Cf-4 resistance protein in Nicotiana benthamiana

生物 细胞生物学 磷酸化 激酶 烟草 蛋白激酶结构域 蛋白激酶A 蛋白质磷酸化 先天免疫系统 信号转导 生物化学 受体 基因 突变体
作者
Wen R.H. Huang,Ciska Braam,Carola Kretschmer,Huan Liu,Filiz Ferik,Aranka M van der Burgh,Max Pluis,Agnes Omabour Hagan,Sergio Landeo Villanueva,Jinbin Wu,Amber van Loosbroek,Yulu Wang,Michael F. Seidl,Johannes Stuttmann,Matthieu H. A. J. Joosten
标识
DOI:10.1101/2023.04.25.538242
摘要

ABSTRACT Cell-surface receptors, which are either receptor-like proteins (RLPs) or receptor-like kinases (RLKs), form the first layer of the plant innate immune system. The LRR-RLKs SUPPRESSOR OF BIR1-1 (SOBIR1) and BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED KINASE 1 (BAK1) are the common regulatory co-receptors of LRR-RLPs. The tomato LRR-RLP Cf-4, which specifically detects the apoplastic effector Avr4 that is secreted by the pathogenic intercellular fungus Cladosporium fulvum , requires SOBIR1 and BAK1 to mediate resistance against this fungus. It is proposed that phosphorylation events take place between the cytoplasmic kinase domains of SOBIR1 and BAK1, which lead to the initiation of various downstream signaling outputs such as the rapid phosphorylation of receptor-like cytoplasmic kinases (RLCKs), the accumulation of reactive oxygen species (ROS), the activation of mitogen-activated protein kinase (MAPK) cascades, and in some cases, the hypersensitive response (HR). Here, by performing in vitro phosphorylation assays, we show that SOBIR1 directly phosphorylates BAK1, whereas BAK1, in its turn, directly phosphorylates SOBIR1, which results in the full activation of the Cf-4-containing immune complex. We found that threonine 522, present in the activation segment of the SOBIR1 kinase domain of Nicotiana benthamiana ( Nb ), is required for its intrinsic kinase activity, and is essential for the Avr4/Cf-4/SOBIR1/BAK1-triggered ROS burst, MAPK activation and the HR. Tyrosine residue Y469 was found to be crucial for the Avr4/Cf-4- triggered activation of MAPKs and the HR, but not for ROS production and Nb SOBIR1 intrinsic kinase activity. RLCKs are well-recognized to act as the initial cytoplasmic transducers, bridging cell-surface receptor complexes with their downstream signaling partners. By knocking out multiple genes belonging to different RLCK-VII subfamilies in N. benthamiana plants stably expressing Cf-4 , we show that members of the RLCK-VII subfamilies 6, 7 and 8 are required for the Avr4/Cf-4-triggered ROS production. Typically, the Avr4 protein triggers a biphasic ROS burst in leaf discs obtained from N. benthamiana:Cf-4 plants, in which the first transitory response is followed by a second, sustained ROS burst. Interestingly, the intensities of the first and second phase of the ROS burst are affected in different ways in the various rlck-vii knock-out plants, indicating that there are different downstream ROS regulatory mechanisms, involving different RLCKs in N. benthamiana . Further studies show that members from RLCK-VII-6, 7 and 8 also play an essential role in regulating the ROS production triggered by flg22, chitin, and the nlp20/RLP23 and the pg13/RLP42 combinations.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
天天快乐应助zhu采纳,获得10
1秒前
IRer79发布了新的文献求助10
1秒前
量子星尘发布了新的文献求助10
1秒前
麦丰完成签到,获得积分10
1秒前
1秒前
likke完成签到,获得积分20
2秒前
2秒前
FashionBoy应助li采纳,获得10
3秒前
3秒前
SciGPT应助薛定谔的猫采纳,获得10
3秒前
4秒前
4秒前
muming完成签到,获得积分20
5秒前
sanlunainiu发布了新的文献求助10
5秒前
林博研完成签到,获得积分20
5秒前
FashionBoy应助风趣的灵枫采纳,获得10
6秒前
7秒前
yihuiqing发布了新的文献求助10
8秒前
likke发布了新的文献求助10
8秒前
嗝嗝发布了新的文献求助10
8秒前
小兔狸花昕完成签到,获得积分20
9秒前
IRer79完成签到,获得积分10
11秒前
彭于晏应助noah采纳,获得10
11秒前
青年才俊发布了新的文献求助10
11秒前
zhu发布了新的文献求助10
12秒前
波波蛋完成签到,获得积分10
12秒前
13秒前
14秒前
15秒前
maguodrgon发布了新的文献求助10
18秒前
科研通AI5应助shell采纳,获得80
18秒前
波波蛋发布了新的文献求助10
18秒前
19秒前
九鹤完成签到,获得积分10
19秒前
jsy完成签到,获得积分10
19秒前
量子星尘发布了新的文献求助10
20秒前
旷野发布了新的文献求助10
20秒前
20秒前
Owen应助浮云521采纳,获得10
20秒前
高分求助中
(应助此贴封号)【重要!!请各用户(尤其是新用户)详细阅读】【科研通的精品贴汇总】 10000
Zeolites: From Fundamentals to Emerging Applications 1500
Architectural Corrosion and Critical Infrastructure 1000
Early Devonian echinoderms from Victoria (Rhombifera, Blastoidea and Ophiocistioidea) 1000
By R. Scott Kretchmar - Practical Philosophy of Sport and Physical Activity - 2nd (second) Edition: 2nd (second) Edition 666
Energy-Size Reduction Relationships In Comminution 500
Principles Of Comminution, I-Size Distribution And Surface Calculations 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 内科学 生物化学 物理 计算机科学 纳米技术 遗传学 基因 复合材料 化学工程 物理化学 病理 催化作用 免疫学 量子力学
热门帖子
关注 科研通微信公众号,转发送积分 4941339
求助须知:如何正确求助?哪些是违规求助? 4207390
关于积分的说明 13077624
捐赠科研通 3986257
什么是DOI,文献DOI怎么找? 2182529
邀请新用户注册赠送积分活动 1198125
关于科研通互助平台的介绍 1110387