Automated hologram reconstruction, including fast defocus correction, for developing time-lapse polychromatic digital holographic microscopy (Conference Presentation)

Polychromatic digital holographic microscopy (P-DHM) has proven its capacity to provide quasi-coherent noise-free quantitative-phase images, allowing a high-quality visualization of cell structure. In this work we propose a fully automated hologram reconstruction methodology, including a fast-numerical approach for the correction of the defocusing resulting from both the axial chromatic aberrations as well as the fluctuations of the optomechanical elements. This methodology able to reconstruct a large number of holograms paves the way to develop a time-resolved P-DHM capable of non-invasively visualizing both the fine cell structure and dynamics.