The controllability of the position and thickness of the compressed layer in the sandwich compression wood is primarily based on the yield stress distribution during a hydrothermal treatment. In this study, the variation law of the yield stress of poplar wood was analyzed in the moisture content (MC) range of absolute dryness to saturation and the temperature range of 30–210℃. A prediction model of wood yield stress response to MC and temperature was developed and the formation of sandwich compression was analyzed. The yield stress of the wood was influenced by the thermal effect and plasticizing effect. In the process of increasing temperature and MC, the yield stress decreased gradually. And the yield stress change rate was 0.29–0.46 MPa in the MC range of 5–10%. The prediction model of wood yield stress was developed by a multiple linear regression analysis. This example demonstrated that coupling distributions of MC and temperature were formed upon heating at 180℃, which led to a gradient distribution of the glass transition temperature and yield stress inside the wood. This phenomenon resulted in the sandwich softening behavior under the coupling of moisture and heat, which was the key formation mechanism of sandwich compressed wood.