大肠杆菌
代谢工程
生物化学
化学
辅因子
苏氨酸
氧化酶试验
大肠杆菌
拉伤
酶
生物
基因
丝氨酸
解剖
作者
Xinxin Liu,Yao Wang,Jianhui Zhang,Yunfeng Lu,Zixing Dong,Chao Yue,Xianqing Huang,Si-Pu Zhang,Dandan Li,Lunguang Yao,Cun‐Duo Tang
标识
DOI:10.1186/s13068-024-02487-4
摘要
Abstract 2,5-Dimethylpyrazine (2,5-DMP) is important pharmaceutical raw material and food flavoring agent. Recently, engineering microbes to produce 2,5-DMP has become an attractive alternative to chemical synthesis approach. In this study, metabolic engineering strategies were used to optimize the modified Escherichia coli BL21 (DE3) strain for efficient synthesis of 2,5-DMP using L -threonine dehydrogenase ( Ec TDH) from Escherichia coli BL21, NADH oxidase ( Eh NOX) from Enterococcus hirae , aminoacetone oxidase ( Sc AAO) from Streptococcus cristatus and L -threonine transporter protein ( Ec SstT) from Escherichia coli BL21, respectively. We further optimized the reaction conditions for synthesizing 2,5-DMP. In optimized conditions, the modified strain can convert L -threonine to obtain 2,5-DMP with a yield of 2897.30 mg/L. Therefore, the strategies used in this study contribute to the development of high-level cell factories for 2,5-DMP. Graphical Abstract
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