Process optimization mitigated the retention loss of an Fc‐fusion protein during ultrafiltration/diafiltration

渗滤 超滤(肾) 化学 色谱法 渗透 浓差极化 大小排阻色谱法 下游加工 融合 微滤 分析化学(期刊) 生物化学 语言学 哲学
作者
Hao Yu,L. Fei
出处
期刊:Biotechnology Progress [Wiley]
标识
DOI:10.1002/btpr.70021
摘要

Abstract In the downstream processing of antibody‐based therapeutics, ultrafiltration/diafiltration (UF/DF) is commonly applied for concentration and buffer exchange in the final formulation. For a given molecule, various factors such as membrane type, feed flux, and transmembrane pressure (TMP) can significantly influence the performance of UF/DF, impacting yield, buffer exchange efficiency, and product quality. Conventional membrane pore size selection is based on product molecular weight to ensure high retention. While working on an Fc‐fusion protein, we found that the pH of load material had a critical effect on the retention of the molecule due to conformational changes at different pH values, as evidenced by the size‐exclusion chromatography (SEC). Meanwhile, optimization of the UF/DF process underscored the importance of concentration polarization to protein retention. Approaches to reduce concentration polarization, such as increasing feed flux and lowering TMP, resulted in less protein loss in the permeate stream. High retention of this Fc‐fusion protein during the UF/DF step can be achieved not only by utilizing a 5 kDa membrane but also by employing a 10 kDa membrane with optimized process parameters such as load conditions, feed flux, and TMP. These observations provide important insights on the factors impacting protein retention beyond the molecular weight cutoff (MWCO) of UF/DF membrane.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
自然水风完成签到 ,获得积分10
刚刚
刚刚
cometx完成签到,获得积分10
1秒前
研友_LOaovZ发布了新的文献求助30
2秒前
yyl完成签到,获得积分10
2秒前
科目三应助yuyu采纳,获得10
2秒前
AOtaku完成签到,获得积分10
2秒前
北方木棉发布了新的文献求助10
3秒前
cometx发布了新的文献求助10
3秒前
5秒前
cherry323完成签到,获得积分10
5秒前
5秒前
6秒前
hahhh7完成签到,获得积分10
6秒前
sun应助黑痴采纳,获得20
6秒前
yibiy完成签到,获得积分10
7秒前
8秒前
qiqi发布了新的文献求助10
9秒前
0h完成签到,获得积分10
9秒前
充电宝应助ljkshr采纳,获得10
9秒前
10秒前
www发布了新的文献求助10
11秒前
12秒前
12秒前
十九完成签到,获得积分10
12秒前
小蘑菇应助XXJ采纳,获得10
12秒前
桕火慢熬完成签到,获得积分10
12秒前
脑洞疼应助开始游戏55采纳,获得10
13秒前
陶陶发布了新的文献求助10
14秒前
xiaoxiaomi应助万木采纳,获得20
14秒前
鱼头关注了科研通微信公众号
14秒前
鱼头关注了科研通微信公众号
15秒前
15秒前
15秒前
言西早发布了新的文献求助20
15秒前
可爱的函函应助猴哥采纳,获得10
15秒前
Pan发布了新的文献求助10
16秒前
科目三应助科研通管家采纳,获得10
16秒前
16秒前
英俊的铭应助科研通管家采纳,获得10
16秒前
高分求助中
Continuum Thermodynamics and Material Modelling 3000
Production Logging: Theoretical and Interpretive Elements 2700
Les Mantodea de Guyane Insecta, Polyneoptera 1000
Conference Record, IAS Annual Meeting 1977 820
England and the Discovery of America, 1481-1620 600
Teaching language in context (Third edition) by Derewianka, Beverly; Jones, Pauline 550
電気学会論文誌D(産業応用部門誌), 141 巻, 11 号 510
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3583231
求助须知:如何正确求助?哪些是违规求助? 3152508
关于积分的说明 9492920
捐赠科研通 2854882
什么是DOI,文献DOI怎么找? 1569373
邀请新用户注册赠送积分活动 735182
科研通“疑难数据库(出版商)”最低求助积分说明 721082