自噬
大戟
生物
病理
医学
细胞生物学
药理学
化学
传统医学
生物化学
细胞凋亡
作者
Ying Yan,Qi Zhou,Xiaoqian Ran,Qing-Yun Lu,Cuishan Zhang,Mingyou Peng,Lei Tang,Rongcan Luo,Ying‐Tong Di,Xiao‐Jiang Hao
摘要
Ten jatrophane diterpenoids were isolated from the whole plant Euphorbia peplus Linn. including seven new ones, named euphjatrophanes A-G (labeled compounds 1, 2, 4–8). Their structures were elucidated with a combination of spectroscopic and single-crystal X-ray crystallography, enabling the identification of compounds 3, 9, and 10 as the previously published euphpepluones G, K, and L, respectively. All compounds were evaluated for their bioactivity with flow cytometry in assays of autophagic flux in HM Cherry-GFP-LC3 (human microglia cells stably expressing the tandem monomeric mCherry-GFP-tagged LC3) cells. Euphpepluone K (9) significantly activated autophagic flux, an effect that was verified with confocal analysis. Moreover, cellular assays showed that euphpepluone K (9) induced autophagy and inhibited Tau pathology.
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