诱导多能干细胞
生物
表观遗传学
干细胞
后生
计算生物学
胚胎干细胞
神经科学
遗传学
基因
DNA甲基化
基因表达
作者
Peter W. Andrews,Ivana Barbaric,Nissim Benvenisty,Jonathan S. Draper,Tenneille E. Ludwig,Florian T. Merkle,Yoji Sato,Claudia Spits,Glyn Stacey,Haoyi Wang,Martín F. Pera
出处
期刊:Cell Stem Cell
[Elsevier]
日期:2022-12-01
卷期号:29 (12): 1624-1636
被引量:38
标识
DOI:10.1016/j.stem.2022.11.006
摘要
It is well established that human pluripotent stem cells (hPSCs) can acquire genetic and epigenetic changes during culture in vitro. Given the increasing use of hPSCs in research and therapy and the vast expansion in the number of hPSC lines available for researchers, the International Society for Stem Cell Research has recognized the need to reassess quality control standards for ensuring the genetic integrity of hPSCs. Here, we summarize current knowledge of the nature of recurrent genetic and epigenetic variants in hPSC culture, the methods for their detection, and what is known concerning their effects on cell behavior in vitro or in vivo. We argue that the potential consequences of low-level contamination of cell therapy products with cells bearing oncogenic variants are essentially unknown at present. We highlight the key challenges facing the field with particular reference to safety assessment of hPSC-derived cellular therapeutics.
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