瓜氨酸化
化学
蛋白质组
蛋白质组学
计算生物学
质谱法
生物化学
翻译后修饰
衍生化
瓜氨酸
氨基酸
酶
生物
基因
色谱法
精氨酸
作者
Yuping Shi,Zihui Li,Bin Wang,Xudong Shi,Hui Ye,Daniel G. Delafield,Langlang Lv,Zhengqing Ye,Zhengwei Chen,Fengfei Ma,Lingjun Li
标识
DOI:10.1021/acs.analchem.2c03844
摘要
Citrullination is a key post-translational modification (PTM) that affects protein structures and functions. Although it has been linked to various biological processes and disease pathogenesis, the underlying mechanism remains poorly understood due to a lack of effective tools to enrich, detect, and localize this PTM. Herein, we report the design and development of a biotin thiol tag that enables derivatization, enrichment, and confident identification of citrullination via mass spectrometry. We perform global mapping of the citrullination proteome of mouse tissues. In total, we identify 691 citrullination sites from 432 proteins which represents the largest data set to date. We discover novel distribution and functions of this PTM. This study depicts a landscape of protein citrullination and lays the foundation for further deciphering their physiological and pathological roles.
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