A Genetic Pathway for Tapetum Development and Function in Arabidopsis

Journal of Integrative Plant BiologyVolume 53, Issue 11 p. 892-900 Research Article A Genetic Pathway for Tapetum Development and Function in Arabidopsis Jun Zhu, Jun Zhu College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China These authors contributed equally to this work.Search for more papers by this authorYue Lou, Yue Lou College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China These authors contributed equally to this work.Search for more papers by this authorXiaofeng Xu, Xiaofeng Xu College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, ChinaSearch for more papers by this authorZhong-Nan Yang, Corresponding Author Zhong-Nan Yang College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China Tel: +86 21 6432 4650; Fax: +86 21 6432 4190; E-mail: [email protected]Search for more papers by this author Jun Zhu, Jun Zhu College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China These authors contributed equally to this work.Search for more papers by this authorYue Lou, Yue Lou College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China These authors contributed equally to this work.Search for more papers by this authorXiaofeng Xu, Xiaofeng Xu College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, ChinaSearch for more papers by this authorZhong-Nan Yang, Corresponding Author Zhong-Nan Yang College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China Tel: +86 21 6432 4650; Fax: +86 21 6432 4190; E-mail: [email protected]Search for more papers by this author First published: 29 September 2011 https://doi.org/10.1111/j.1744-7909.2011.01078.xCitations: 112 Available online on 29 September 2011 at http://www.jipb.net and http://www.wileyonlinelibrary.com/journal/jipb Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract In anther development, tapetal cells take part in complex processes, including endomitosis and apoptosis (programmed cell death). The tapetum provides many of the proteins, lipids, polysaccharides and other molecules necessary for pollen development. Several transcription factors, including DYT1, TDF1, AMS, MS188 and MS1, have been reported to be essential for tapetum development and function in Arabidopsis thaliana. Here, we present a detailed cytological analysis of knockout mutants for these genes, along with an in situ RNA hybridization experiment and double mutant analysis showing that these transcription factors form a genetic pathway in tapetum development. DYT1, TDF1 and AMS function in early tapetum development, while MS188 and MS1 are important for late tapetum development. The genetic pathway revealed in this work facilitates further investigation of the function and molecular mechanisms of tapetum development in Arabidopsis. Citing Literature Supporting Information Figure S1. Anther sections of wild-type and dyt1 at stage 3. Figure S2. Flow diagram and genotyping for double mutant construction. Table S1. Primers used for double mutant identification. Table S2. Primers used for in situ hybridization. Filename Description JIPB_1078_sm_TableS1.xls8.5 KB Supporting info item JIPB_1078_sm_TableS2.xls8.5 KB Supporting info item JIPB_1078_sm_FigureS1.tif523.8 KB Supporting info item JIPB_1078_sm_FigureS2.tif365.8 KB Supporting info item Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume53, Issue11November 2011Pages 892-900 RelatedInformation