生物正交化学
点击化学
化学
体内
生物化学
组合化学
叠氮化物
标签
作者
Borja Belda-Palazón,Alejandro Ferrando,Rosa Farràs
出处
期刊:Methods of Molecular Biology
日期:2016-01-01
卷期号:1449: 369-382
标识
DOI:10.1007/978-1-4939-3756-1_24
摘要
The development of novel bioorthogonal reactives that can be used to tag biomolecules in vivo has revolutionized the studies of cellular and molecular biology. Among those novel reactive substances, amino acid analogs can be used to label nascent proteins, thus opening new avenues for measuring protein translation rates in vivo with a limited manipulation of the sample. Here, we describe the use of Click-chemistry to tag and separate newly synthesized proteins in mammalian cells that can be used, coupled with western analysis, to estimate the translation rate of any protein of interest.
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