Activation of transient receptor potential vanilloid 1 by lipoxygenase metabolites depends on PKC phosphorylation

TRPV1型 瞬时受体电位通道 G蛋白偶联受体 细胞生物学 磷酸化 化学 蛋白激酶C 信号转导 受体 生物 生物化学
作者
Rakesh Kumar,Adina Hazan,Matan Geron,Rebbeca Steinberg,Lital Livni,Henry Matzner,Avi Priel
出处
期刊:The FASEB Journal [Wiley]
卷期号:31 (3): 1238-1247 被引量:24
标识
DOI:10.1096/fj.201601132r
摘要

Peripheral neuronal activation by inflammatory mediators is a multifaceted physiological response that involves a multitude of regulated cellular functions. One key pathway that has been shown to be involved in inflammatory pain is Gq/GPCR, whose activation by inflammatory mediators is followed by the regulated response of the cation channel transient receptor potential vanilloid 1 (TRPV1). However, the mechanism that underlies TRPV1 activation downstream of the Gq/GPCR pathway has yet to be fully defined. In this study, we employ pharmacological and molecular biology tools to dissect this activation mechanism via perforated-patch recordings and calcium imaging of both neurons and a heterologous system. We showed that TRPV1 activity downstream of Gq/GPCR activation only produced a subdued current, which was noticeably different from the robust current that is typical of TRPV1 activation by exogenous stimuli. Moreover, we specifically demonstrated that 2 pathways downstream of Gq/GPCR signaling, namely endovanilloid production by lipoxygenases and channel phosphorylation by PKC, converge on TRPV1 to evoke a tightly regulated response. Of importance, we show that only when both pathways are acting on TRPV1 is the inflammatory-mediated response achieved. We propose that the requirement of multiple signaling events allows subdued TRPV1 activation to evoke regulated neuronal response during inflammation.—Kumar R., Hazan, A., Geron, M., Steinberg, R., Livni, L., Matzner, H., Priel, A. Activation of transient receptor potential vanilloid 1 by lipoxygenase metabolites depends on PKC phosphorylation. FASEB J. 31, 1238–1247 (2017). www.fasebj.org
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
科研通AI2S应助风枫叶采纳,获得10
1秒前
雨辰发布了新的文献求助10
1秒前
2秒前
2秒前
小敏完成签到,获得积分10
3秒前
3秒前
喵茸茸完成签到,获得积分10
3秒前
10发布了新的文献求助10
3秒前
拉长的元芹完成签到,获得积分10
4秒前
田様应助空曲采纳,获得10
4秒前
xinC完成签到 ,获得积分10
4秒前
xiaohan,JIA完成签到,获得积分10
4秒前
小圆圈发布了新的文献求助10
7秒前
天天快乐应助zhuzhu采纳,获得10
7秒前
华仔应助fujun0095采纳,获得10
8秒前
Chrisiu发布了新的文献求助10
8秒前
Owen应助147258采纳,获得10
9秒前
殇璃完成签到 ,获得积分10
11秒前
12秒前
一路有你完成签到 ,获得积分10
12秒前
12秒前
13秒前
溪谷完成签到,获得积分10
14秒前
14秒前
14秒前
羊青丝完成签到,获得积分10
15秒前
CodeCraft应助xiao123789采纳,获得10
16秒前
Chrisiu完成签到,获得积分20
17秒前
17秒前
jw完成签到,获得积分10
17秒前
小蘑菇应助jeep先生采纳,获得10
17秒前
18秒前
黙宇循光发布了新的文献求助10
18秒前
赘婿应助Fin2046采纳,获得10
19秒前
redamancy完成签到 ,获得积分10
20秒前
20秒前
啦啦啦完成签到,获得积分10
23秒前
aaaaa发布了新的文献求助10
23秒前
23秒前
莫友安完成签到,获得积分10
23秒前
高分求助中
The Oxford Handbook of Social Cognition (Second Edition, 2024) 1050
Kinetics of the Esterification Between 2-[(4-hydroxybutoxy)carbonyl] Benzoic Acid with 1,4-Butanediol: Tetrabutyl Orthotitanate as Catalyst 1000
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
юрские динозавры восточного забайкалья 800
English Wealden Fossils 700
Handbook of Qualitative Cross-Cultural Research Methods 600
Chen Hansheng: China’s Last Romantic Revolutionary 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3139996
求助须知:如何正确求助?哪些是违规求助? 2790894
关于积分的说明 7796961
捐赠科研通 2447258
什么是DOI,文献DOI怎么找? 1301779
科研通“疑难数据库(出版商)”最低求助积分说明 626340
版权声明 601194