基因敲除
癌症研究
细胞生长
染色质免疫沉淀
细胞周期
长非编码RNA
细胞周期蛋白D1
EZH2型
小干扰RNA
细胞
生物
下调和上调
化学
细胞培养
染色质
基因表达
转染
基因
生物化学
发起人
遗传学
作者
Cong Wang,Lin Mou,Hai-Xia Chai,Feng Wang,Yuchen Yin,Xiaoyu Zhang
标识
DOI:10.1016/j.biopha.2017.01.031
摘要
Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. Recent evidences have demonstrated that long non-coding RNAs (lncRNAs) act as key regulators of tumor development and progression including HCC. In the study, we showed that the expression level of HNF1A-AS1 was up-regulated in HCC cell lines. Furthermore, CCK-8 cell proliferation assays and cell cycle analysis showed that HNF1A-AS1 over-expression facilitated HCC cell proliferation by promoting the cell proliferation and S-phase progression, whereas HNF1A-AS1 knockdown had the opposite effect. Western-blotting analysis revealed that knockdown of HNF1A-AS1 inhibited the cycle-relative protein cyclin-D1 and PCNA expression in HCC cells. Mechanism, RNA immunoprecipitation (RIP) and Chromatin immunoprecipitation (ChIP) assays showed that by interacting with enhancer of zeste homolog 2 (EZH2), HNF1A-AS1 promoted HCC cell proliferation by repressing the NKD1 and p21 expression. These results suggested that HNF1A-AS1 may contribute to HCC progression, which may be an effective therapeutic target for patients.
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