Hyaluronic acid hydrogels cross-linked by polyethylene glycol diglycidyl ether (PEGDE) for long-lasting dermal filler applications

Event Abstract Back to Event Hyaluronic acid hydrogels cross-linked by polyethylene glycol diglycidyl ether (PEGDE) for long-lasting dermal filler applications Ho-Yong Lee1*, Seol-Ha Jeong1, Jae-Uk Baek1, Ju-Ha Song1 and Hyoun-Ee Kim1 1 Seoul national university, Materials science and engineering, Korea Statement of Purpose: Dermal fillers have been widely used for cosmetic applications such as wrinkle management and plastic surgery. Hyaluronic acid (HA) has been regarded as the best candidate for dermal fillers due to its non-immunogenicity and biodegradability[1]. HA for commercial dermal fillers is often chemically cross-linked by 1,4-butanediol diglycidyl ether (BDDE) for enhanced mechanical properties and biostability[2]. However, BDDE-crosslinked HA fillers is known to lose their mechanical integrity within 6 months in vivo by enzymatic degradation[3]. Therefore, in this study, we have incorporated polyethylene glycol diglycidyl ether (PEGDE) into HA hydrogels as a chemical cross-linker to further enhance mechanical and biological stability as compare to BDDE. Moreover, we investigate in vitro and in vivo biological behavior of PEGDE-crosslinked HA fillers. Methods: HA hydrogels were cross-linked using PEGDE (Mn = 2000 Da) or BDDE. 20 wt. % HA solution was prepared by dissolving 0.1 g HA (Mn = 2.2 MDa) in 0.5 mL 0.3 N NaOH aqueous solution. 5 µL BDDE was added to the HA solution. Likewise, the molar equivalent amount (0.15 mol crosslinker/ 1 mol HA) of PEGDE to BDDE was added to the HA solution. The reactions were performed at 25 oC for 24 h. Rheology measurement was carried out using parallel plate rheometer. Swelling test was performed with fully swollen samples in PBS at 37 ºC. In vitro degradation was performed in Hyaluronidase/PBS (100 U/ml) solution. In vitro cell test was performed by culturing L929 fibroblasts on the gel surface. Results: Rheological behavior of cross-linked HA hydrogels by BDDE and PEGDE were shown in Fig. 1a. The storage modulus of PEGDE-cross-linked HA hydrogel was higher than that of BDDE-crosslinked HA hydrogel, meaning higher elasticity in PEGDE-cross-linked HA hydrogel. Swelling behavior was inversely correlated to storage modulus (Fig. 1b). Degradation stability by hyaluronidase was evaluated by volumetric and shape change, showing PEGDE-cross-linked HA hydrogel is more resistant to degradation than BDDE-cross-linked HA hydrogel (Fig. 2). Between time intervals, the hydrogels maintained their initial shape. Cell spreading was observed for both gels (Fig. 3). On PEGDE-cross-linked HA hydrogel, fibroblasts spread well almost "spindle-like" phenotype, whereas "spherical" shape on BDDE-cross-linked HA hydrogel, implying cell-matrix interaction is higher on PEGDE-cross-linked HA hydrogel surface. Conclusions: PEGDE-cross-linked HA hydrogel was fabricated with more efficient cross-linking effect as compared to BDDE-cross-linked HA hydrogel. Higher elasticity and biostability can be obtained by using PEGDE rather than BDDE. Moreover, the hydrogel can controllable in mechanical properties and degradability by the amount of cross-linker with minimally modified. Exploiting great biocompatibility of PEG/HA hydrogel can be a good candidate for long-lasting dermal filler applications. Small and Medium Business Administration, Republic of Korea (S2174750)References:[1] S. Dastoor et al. J Oral Implantol 2007; 33(4):191-204.[2] C.E. Schante et al. Carbohydrate Polymers 85 2011; 469–489[3] A. Tezel et al. J. Cosmet. Laser Ther. 2008; 10, 35–42. Keywords: Hydrogel, biomedical application, medical application, biodegredation Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Naturally-derived materials and biopolymers Citation: Lee H, Jeong S, Baek J, Song J and Kim H (2016). Hyaluronic acid hydrogels cross-linked by polyethylene glycol diglycidyl ether (PEGDE) for long-lasting dermal filler applications. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.01809 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers' terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. * Correspondence: Dr. Ho-Yong Lee, Seoul national university, Materials science and engineering, Seoul, Korea, Email1 Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Ho-Yong Lee Seol-Ha Jeong Jae-Uk Baek Ju-Ha Song Hyoun-Ee Kim Google Ho-Yong Lee Seol-Ha Jeong Jae-Uk Baek Ju-Ha Song Hyoun-Ee Kim Google Scholar Ho-Yong Lee Seol-Ha Jeong Jae-Uk Baek Ju-Ha Song Hyoun-Ee Kim PubMed Ho-Yong Lee Seol-Ha Jeong Jae-Uk Baek Ju-Ha Song Hyoun-Ee Kim Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.