生物催化
化学
细胞内
纳米技术
催化作用
自组装
材料科学
生物化学
离子液体
作者
Jiakang Tang,Ji Liu,Qizhen Zheng,Wenting Li,Jinhan Sheng,Lanqun Mao,Ming Wang
标识
DOI:10.1002/anie.202105634
摘要
Abstract Hydrogen‐bonded organic frameworks (HOFs) are porous materials with great potential for biological applications. The self‐assembly of HOFs and biomacromolecules, however, is challenging. We report herein the self‐assembly of nanoscale HOFs (nHOFs) to encapsulate protein for intracellular biocatalysis. The self‐assembly of tetrakis(4‐amidiniumphenyl)methane and azobenzenedicarboxylate can encapsulate protein in situ to form protein@nHOFs under mild conditions. This strategy is applicable to proteins with different surface charge and molecular weight, showing a high protein encapsulation efficiency and minimal effect on protein activity. A cellular delivery study shows that the protein@TA‐HOFs can efficiently enter cells and retain enzyme activity for biochemical catalysis in living cells for neuroprotection. Our strategy paves new avenues for interfacing nHOFs with biological settings and sheds light on expanding nHOFs as a platform for biomacromolecule delivery and disease treatment.
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