Effect of probiotics supplementation on growth,nutrient metabolism and antioxidant capacity of Litopenaeus vannamei

【Objective】 A 28-day experiment was conducted to investigate the effects of Bacillus licheniformis and Bacillus coagulans on growth performance,nutrient metabolism and antioxidant capacity of Litopenaeus vannamei.【Method】 L.vannamei were randomly allocated to 3 groups,those in the control group were fed with a basal diet wrapped with albumen,and those in the experimental groups were fed with basal diets supplemented with B. coagulans(10~8 CFU/g) and B. licheniformis(10~8 CFU/g),respectively.Samples were taken on the 7 th,14 th,21 st and 28 th days of the experiment.The contents of total protein(TP),total cholesterol(TC),triglyceride(TG) and nitric oxide(NO) and the activities of glutamine synthetase(GS),malic dehydrogenase(MDH),lipase(LPS),hexokinase(HK),glutathione peroxidase(GSH-Px),catalase(CAT),superoxide dismutase(SOD),nitric oxide synthetase(NOS) and total antioxidant(T-AOC) were measured.At the end of the experiment,the growth performance and muscle nutrients of L. vannamei were measured.【Result】(1) Compared with the control group,the final weight increased significantly and the feed conversion ratio decreased(P 0.05).(3) The activity of T-AOC was the highest in B. coagulans group(55.21 U/mL) on the 7 th day and in B. licheniformis group(45.08 U/mL) on the 14 th day with significant differences with the control group(P 0.05).The activity of GSH-Px in B. coagulans group reached the highest level on the 28 th day(957.30 U/mL),while that in B. licheniformis group reached the highest level on the 21 st day(932.25 U/mL),and they were significant than that in the control group(P<0.05).The activity of NOS and the content of NO in B. coagulans group reached the highest levels on the 14 th day,and they were significantly higher than that in the control group(P<0.05).The content of NO in B. licheniformis group reached the highest level on the 28 th day,and it was significantly higher than in the control group(P<0.05).【Conclusion】 Adding B. licheniformis and B. coagulans in diet could improve the growth performance,nutrient metabolism level,oxygen metabolism and antioxidant capacity of L. vannamei.