神经再支配
坐骨神经
医学
再生(生物学)
坐骨神经损伤
腓肠肌
周围神经损伤
挤压伤
神经损伤
麻醉
解剖
脊髓损伤
腰脊髓
复合肌肉动作电位
脊髓
外科
电生理学
生物
内科学
骨骼肌
细胞生物学
背
精神科
作者
Zhonghai Huang,Ai-Ying Feng,Jing Liu,Libing Zhou,Bing Zhou,Panpan Yu
标识
DOI:10.4103/1673-5374.313054
摘要
Inhibitor of DNA binding 2 (Id2) can promote axonal regeneration after injury of the central nervous system. However, whether Id2 can promote axonal regeneration and functional recovery after peripheral nerve injury is currently unknown. In this study, we established a mouse model of bilateral sciatic nerve crush injury. Two weeks before injury, AAV9-Id2-3×Flag-GFP was injected stereotaxically into the bilateral ventral horn of lumbar spinal cord. Our results showed that Id2 was successfully delivered into spinal cord motor neurons projecting to the sciatic nerve, and the number of regenerated motor axons in the sciatic nerve distal to the crush site was increased at 2 weeks after injury, arriving at the tibial nerve and reinnervating a few endplates in the gastrocnemius muscle. By 1 month after injury, extensive neuromuscular reinnervation occurred. In addition, the amplitude of compound muscle action potentials of the gastrocnemius muscle was markedly recovered, and their latency was shortened. These findings suggest that Id2 can accelerate axonal regeneration, promote neuromuscular reinnervation, and enhance functional improvement following sciatic nerve injury. Therefore, elevating the level of Id2 in adult neurons may present a promising strategy for peripheral nerve repair following injury. The study was approved by the Experimental Animal Ethics Committee of Jinan University (approval No. 20160302003) on March 2, 2016.
科研通智能强力驱动
Strongly Powered by AbleSci AI