检出限
胶体金
分析物
核酸
链霉亲和素
纳米技术
生物分子
分析灵敏度
纳米颗粒
碳纳米颗粒
线性范围
灵敏度(控制系统)
化学
色谱法
材料科学
生物素
生物化学
医学
工程类
病理
替代医学
电子工程
作者
Juan Carlos Porras,Mireia Bernuz,Jennifer Marfa,Arnau Pallarès-Rusiñol,Mercè Martı́,María Isabel Pividori
出处
期刊:Nanomaterials
[Multidisciplinary Digital Publishing Institute]
日期:2021-03-15
卷期号:11 (3): 741-741
被引量:29
摘要
A lateral flow assay (LFA) is a paper-based, point-of-need test designed to detect a specific analyte in complex samples in low-resource settings. Although LFA has been successfully used in different applications, its use is still limited when high sensitivity is required, especially in the diagnosis of an early-stage condition. The limit of detection (LOD) is clearly related to the signal-generating system used to achieve the visual readout, in many cases involving nanoparticles coupled to a biomolecule, which, when combined, provides sensitivity and specificity, respectively. While colloidal gold is currently the most-used label, other detection systems are being developed. Carbon nanoparticles (CNPs) demonstrate outstanding features to improve the sensitivity of this technology by producing an increased contrast in the paper background. Based on the necessity of sensitivity improvement, the aim of this work is a comparative study, in terms of analytical performance, between commercial streptavidin gold nanoparticles (streptAv-AuNPs) and avidin carbon nanoparticles (Av-CNPs) in a nucleic acid lateral flow assay. The visual LOD of the method was calculated by serial dilution of the DNA template, ranging from 0.0 to 7 pg μL−1/1.5 × 104 CFU mL−1). The LFA achieved visual detection of as low as 2.2 × 10−2 pg μL−1 using Av-CNPs and 8.4 × 10−2 pg μL−1 using streptAv-AuNPs. These LODs could be obtained without the assistance of any instrumentation. The results demonstrate that CNPs showed an increased sensitivity, achieving the nanomolar range even by visual inspection. Furthermore, CNPs are the cheapest labels, and the suspensions are very stable and easy to modify.
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