球形红杆菌
生物化学
辅酶Q10
代谢工程
生物合成
代谢途径
酶
化学
焊剂(冶金)
红杆菌属
ATP合酶
辅因子
生物
光合作用
突变体
基因
有机化学
作者
Wenqiang Lu,Lidan Ye,Xiaomei Lv,Wenping Xie,Jiali Gu,Zhaofeng Chen,Yujun Zhu,Aipeng Li,Hongwei Yu
标识
DOI:10.1016/j.ymben.2015.03.012
摘要
In this report, UbiE and UbiH in the quinone modification pathway (QMP) were identified in addition to UbiG as bottleneck enzymes in the CoQ10 biosynthesis by Rhodobacter sphaeroides. The CoQ10 content was enhanced after co-overexpression of UbiE and UbiG, however, accompanied by the accumulation of the intermediate 10P-MMBQ. UbiH was then co-overexpressed to pull the metabolic flux towards downstream, resulting in an elevated CoQ10 productivity and decreased biomass. On the other hand, the expression levels of UbiE and UbiG were tuned to eliminate the intermediate accumulation, however at the sacrifice of productivity. To alleviate the detrimental effect on either productivity or cell growth, we tried to fuse UbiG with UbiE and localize them onto the membrane to elevate intermediate conversion. By fusing UbiE and UbiG to pufX, CoQ10 was accumulated to 108.51±2.76mg/L with a biomass of 12.2±0.9g/L. At last, we combined the optimized QMP and the previously engineered 2-methyl-d-erythritol-4-phosphate pathway (MEP) to further boost CoQ10 biosynthesis, resulting in a strain with 138±2.64mg/L CoQ10 production.
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