生物
突变体
基因
次生代谢
遗传学
巢状曲霉
镰刀菌
转录组
转录因子
表型
分生孢子
抄写(语言学)
细胞生物学
基因表达
哲学
生物合成
语言学
作者
Erik Lysøe,Matias Pasquali,Andrew Breakspear,Harold Kistler
标识
DOI:10.1094/mpmi-03-10-0075
摘要
Fusarium graminearum is an important plant-pathogenic fungus and the major cause of cereal head blight. Here, we report the functional analysis of FgStuA, the gene for a transcription factor with homology to key developmental regulators in fungi. The deletion mutant was greatly reduced in pathogenicity on wheat heads and in production of secondary metabolites. Spore production was significantly impaired in ΔFgStuA, which did not develop perithecia and sexual ascospores, and lacked conidiophores and phialides, leading to delayed production of aberrant macroconidia. FgStuAp appears to act as a global regulator that may affect many diverse aspects of the life cycle of F. graminearum. Transcriptome analysis shows that thousands of genes are differentially expressed in the mutant during asexual sporulation and infection of wheat heads and under conditions that induce secondary metabolites, including many that could account for the mutant phenotypes observed. The primary regulatory targets of FgStuAp are likely genes involved in cell-cycle control, and the predicted FgStuAp sequence has an APSES domain, with homology to helix-loop-helix proteins involved in cell-cycle regulation. The Aspergillus StuAp response element (A/TCGCGT/ANA/C) was found highly enriched in the promoter sequences of cell-cycle genes, which was upregulated in the ΔFgStuA deletion mutant.
科研通智能强力驱动
Strongly Powered by AbleSci AI