TRPV1型
瞬时受体电位通道
锚定
受体
化学
细胞生物学
辣椒素
转染
锚蛋白重复序列
生物化学
生物
基因
作者
Laura R. Sadofsky,Koti Sreekrishna,Yong Lin,Renee Schinaman,Kate Gorka,Yogita Mantri,John Christian Haught,Thomas G. Huggins,Robert J. Isfort,Charles C. Bascom,Alyn H. Morice
出处
期刊:Cells
[MDPI AG]
日期:2014-06-11
卷期号:3 (2): 616-626
被引量:34
摘要
Transient receptor potential (TRP) ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1) receptors are implicated in modulation of cough and nociception. In vivo, TRPA1 and TRPV1 are often co-expressed in neurons and TRPA1V1 hetero-tetramer formation is noted in cells co-transfected with the respective expression plasmids. In order to understand the impact of TRP receptor interaction on activity, we created stable cell lines expressing the TRPA1, TRPV1 and co-expressing the TRPA1 and TRPV1 (TRPA1V1) receptors. Among the 600 compounds screened against these receptors, we observed a number of compounds that activated the TRPA1, TRPV1 and TRPA1V1 receptors; compounds that activated TRPA1 and TRPA1V1; compounds that activated TRPV1 and TRPA1V1; compounds in which TRPA1V1 response was modulated by either TRPA1 or TRPV1; and compounds that activated only TRPV1 or TRPA1 or TRPA1V1; and one compound that activated TRPA1 and TRPV1, but not TRPA1V1. These results suggest that co-expression of TRPA1 and TRPV1 receptors imparts unique activation profiles different from that of cells expressing only TRPA1 or TRPV1.
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