二金字塔
色谱法
气相色谱法
化学
氮气
医学
有机化学
内科学
作者
James F. Brien,Kanji Nakatsu,Paul W. Armstrong
出处
期刊:Journal of Pharmacological Methods
[Elsevier]
日期:1983-06-01
卷期号:9 (4): 295-308
被引量:4
标识
DOI:10.1016/0160-5402(83)90069-4
摘要
A rapid, reliable assay is described for the quantitation of disopyramide (DP) and its metabolite, mono-N-desisopropyl-disopyramide (MNDP), in serum using p-chloro-disopyramide as the internal standard and aqueous standards for calibration. The procedure involves extraction of the serum sample with diethyl ether, derivatization of MNDP with acetic anhydride, and gas-liquid Chromatographic analysis with nitrogen-selective detection. The accuracy of the procedure is97.8 ± 10.3 (SD)% for DP over the concentration range, 0.25–10.00 μg/ml (n = 37), and 99.6 ± 10.2 (SD)% for MNDP over the concentration range, 0.50–5.00 μg/ml (n = 18). The within-day coefficient of variation is less than 8% for DP and 12% for MNDP. The lower limit of quantitative sensitivity is 0.25 μg/ml for DP and 0.50 μg/ml for MNDP, and the lower limit of detection is 0.6 ng and 1.6 ng, respectively, for DP and MNDP. This assay has been used to determine the time-course of DP and MNDP and the protein binding of DP (without requiring an in vitro protein binding curve) in the serum obtained from patients and laboratory animals during DP administration.
科研通智能强力驱动
Strongly Powered by AbleSci AI