透明质酸酶
医学
水肿
体内
透明质酸
麻醉
外科
酶
生物化学
解剖
化学
生物
生物技术
作者
Tara Nekoroski,Rudolph D. Paladini,Daniel N. Sauder,Gary Frost,Gilbert‐A. Keller
摘要
Abstract Background Edema commonly accompanies surgical procedures and when excessive, can adversely affect surgical outcomes. The skin extracellular matrix, including one of its primary components, hyaluronan ( HA ), is a significant barrier to effective drainage of accumulated edematous fluid. Recombinant human hyaluronidase (r H u PH 20) is a human hyaluronidase that acts transiently and locally to depolymerize HA . A non‐liposomal gel formulation that provides a sustained release of r H u PH 20 was tested in vivo in a preclinical murine model of acquired lymphedema. Methods Lymphedemic mice were injected 24 hours before surgery, and at 2 and 12 days following surgery with r H u PH 20 sustained release gel ( PH 20 SR gel). Quantitative assessment of treatment response indicated that a single dose of PH 20 SR gel resulted in accelerated resolution and reduced severity of post‐surgical edema as compared to the gel vehicle (control). Results Statistically significant enzymatic degradation of HA was demonstrated up to 5 mm from the injection site, and histological analysis confirmed removal of HA up to 72 hours following PH 20 SR gel administration. Conclusions These results demonstrate sustained hyaluronidase enzymatic activity that promotes diffusion of accumulated post‐surgical edematous fluid, suggesting that PH 20 SR gel may be a useful adjuvant in promoting postoperative edema resolution.
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