摘要
Journal of Biomedical Materials ResearchVolume 44, Issue 2 p. 130-139 Human plasma fibrinogen adsorption and platelet adhesion to polystyrene Wei-Bor Tsai, Wei-Bor Tsai Department of Bioengineering, Box 351750, University of Washington, Seattle, Washington 98195-1750Search for more papers by this authorJohn M. Grunkemeier, John M. Grunkemeier Department of Bioengineering, Box 351750, University of Washington, Seattle, Washington 98195-1750Search for more papers by this authorThomas A. Horbett, Corresponding Author Thomas A. Horbett Department of Bioengineering, Box 351750, University of Washington, Seattle, Washington 98195-1750 Department of Chemical Engineering, Box 351750, University of Washington, Seattle, Washington 98195-1750Departments of Bioengineering, and Chemical Engineering, Box 351750, University of Washington, Seattle, Washington 98195-1750Search for more papers by this author Wei-Bor Tsai, Wei-Bor Tsai Department of Bioengineering, Box 351750, University of Washington, Seattle, Washington 98195-1750Search for more papers by this authorJohn M. Grunkemeier, John M. Grunkemeier Department of Bioengineering, Box 351750, University of Washington, Seattle, Washington 98195-1750Search for more papers by this authorThomas A. Horbett, Corresponding Author Thomas A. Horbett Department of Bioengineering, Box 351750, University of Washington, Seattle, Washington 98195-1750 Department of Chemical Engineering, Box 351750, University of Washington, Seattle, Washington 98195-1750Departments of Bioengineering, and Chemical Engineering, Box 351750, University of Washington, Seattle, Washington 98195-1750Search for more papers by this author First published: 29 January 1999 https://doi.org/10.1002/(SICI)1097-4636(199902)44:2<130::AID-JBM2>3.0.CO;2-9Citations: 274AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract The purpose of this study was to further investigate the role of fibrinogen adsorbed from plasma in mediating platelet adhesion to polymeric biomaterials. Polystyrene was used as a model hydrophobic polymer; i.e., we expected that the role of fibrinogen in platelet adhesion to polystyrene would be representative of other hydrophobic polymers. Platelet adhesion was compared to both the amount and conformation of adsorbed fibrinogen. The strategy was to compare platelet adhesion to surfaces preadsorbed with normal, afibrinogenemic, and fibrinogen-replenished afibrinogenemic plasmas. Platelet adhesion was determined by the lactate dehydrogenase (LDH) method, which was found to be closely correlated with adhesion of 111In-labeled platelets. Fibrinogen adsorption from afibrinogenemic plasma to polystyrene (Immulon I®) was low and <10 ng/cm2. Platelet adhesion was absent on surfaces preadsorbed with afibrinogenemic plasma when the residual fibrinogen was low enough (<60 μg/mL). Platelet adhesion was restored on polystyrene preadsorbed with fibrinogen-replenished afibrinogenemic plasma. Addition of even small, subnormal concentrations of fibrinogen to afibrinogenemic plasma greatly increased platelet adhesion. In addition, surface-bound fibrinogen's ability to mediate platelet adhesion was different, depending on the plasma concentration from which fibrinogen was adsorbed. These differences correlated with changes in the binding of a monoclonal antibody that binds to the Aα chain RGDS (572–575), suggesting alteration in the conformation or orientation of the adsorbed fibrinogen. Platelet adhesion to polystyrene preadsorbed with blood plasma thus appears to be a strongly bivariate function of adsorbed fibrinogen, responsive to both low amounts and altered states of the adsorbed molecule. © 1999 John Wiley & Sons, Inc. 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