多形体
核糖体
信使核糖核酸
核糖体RNA
化学
蛋白质生物合成
翻译(生物学)
核糖体蛋白
生物化学
单体
蔗糖
核糖核酸
生物
基因
聚合物
有机化学
作者
Maria C. Rivera,Bruce A. Maguire,James A. Lake
出处
期刊:CSH Protocols
[Cold Spring Harbor Laboratory]
日期:2015-03-01
卷期号:2015 (3): pdb.prot081364-pdb.prot081364
被引量:7
标识
DOI:10.1101/pdb.prot081364
摘要
Isolated polysomes (also known as translating ribosomes or polyribosomes) are mRNA-ribosome complexes that are frequently used for the in vitro study of the regulation of protein synthesis. Here we describe a protocol for the isolation of prokaryotic and plant polysomes by sucrose gradient sedimentation. The protocol allows for the separation of multiple ribosomes attached to mRNA from run-off ribosome monomers. In addition to collecting the polysome pellet, if the sucrose gradients used in this protocol are fractionated, it is possible to isolate and quantify the polysomes, the ribosome monomers, and the ribosomal subunits.
科研通智能强力驱动
Strongly Powered by AbleSci AI