锚蛋白重复序列
锚定
基因
生物
基因家族
污渍
分子生物学
免疫染色
遗传学
基因表达
保守序列
幽灵蛋白
蛋白质-蛋白质相互作用
蛋白质结构域
蛋白质家族
蛋白质结构
细胞生物学
膜蛋白
蛋白质折叠
肽序列
免疫学
免疫组织化学
作者
Yun Zhu,Naoto Kakinuma,Yong Wang,Ryoiti Kiyama
标识
DOI:10.1016/j.bbagen.2007.09.017
摘要
The human Kank gene was found as a candidate tumor suppressor for renal cell carcinoma, and encodes an ankyrin-repeat domain-containing protein, Kank. Here, we report a new family of proteins consisting of three Kank (Kank1)-associated members, Kank2, Kank3 and Kank4, which were found by domain and phylogenetic analyses. Besides the conserved ankyrin-repeat and coiled-coil domains, there was a conserved motif at the N-terminal (KN motif) containing potential motifs for nuclear localization and export signals. Gene expression of these genes was examined by RT-PCR at the mRNA level and by Western blotting and immunostaining at the protein level. Kank family genes showed variations in the expression level among tissues and kidney cell lines. Furthermore, the results of overexpression of these genes in NIH3T3 cells suggest that all of these family proteins have an identical role in the formation of actin stress fibers.
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