化学
发酵
青霉素
萃取(化学)
色谱法
水溶液
溶剂
双水相体系
食品科学
有机化学
抗生素
生物化学
作者
Yi‐Xin Guan,Ziqiang Zhu,Lehe Mei
标识
DOI:10.1080/01496399608001067
摘要
Abstract A new attempt was made to reform the original penicillin recovery technology, and fundamental data were provided for further scale-up. The volume of fermentation broth processed was 1000 mL, the mass of penicillin crystal obtained was 7.228 g with an average purity of 84.15%, the overall recovery ratio of aqueous two-phase partitioning (ATPP) technology was 76.56%, and the entire technical process is described. Compared to the original organic solvent extraction, ATPP technology can extract penicillin from whole fermentation broth directly. The pH should be adjusted only once and the activity of penicillin can be improved. The extraction steps are decreased from three times to one, and the extraction technology is integrated. A new field is opened up by this powerful ATPP technology. Key Words: Aqueous two-phase partitioningPenicillinLiquid-liquid equilibriumExtractive fermentation
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