THP1细胞系
巨噬细胞
免疫系统
表型
蛋白质组学
细胞生物学
生物
计算生物学
分子生物学
化学
体外
细胞培养
免疫学
遗传学
基因
作者
Pengfei Li,Chen Ma,Jia Li,Shanshan You,Liuyi Dang,Jingyu Wu,Hao Zou,Jun Li,Yuan Zhi,Lin Chen,Shisheng Sun
出处
期刊:Journal of Zhejiang University-science B
[Springer Nature]
日期:2022-05-01
卷期号:23 (5): 407-422
被引量:28
标识
DOI:10.1631/jzus.b2100930
摘要
Macrophages are widely distributed immune cells that contribute to tissue homeostasis. Human THP-1 cells have been widely used in various macrophage-associated studies, especially those involving pro-inflammatory M1 and anti-inflammatory M2 phenotypes. However, the molecular characterization of four M2 subtypes (M2a, M2b, M2c, and M2d) derived from THP-1 has not been fully investigated. In this study, we systematically analyzed the protein expression profiles of human THP-1-derived macrophages (M0, M1, M2a, M2b, M2c, and M2d) using quantitative proteomics approaches. The commonly and specially regulated proteins of the four M2 subtypes and their potential biological functions were further investigated. The results showed that M2a and M2b, and M2c and M2d have very similar protein expression profiles. These data could serve as an important resource for studies of macrophages using THP-1 cells, and provide a reference to distinguish different M2 subtypes in macrophage-associated diseases for subsequent clinical research.
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