生物分子
材料科学
胞浆
纳米技术
表面电荷
内吞循环
生物高聚物
聚合物
胶束
纳米颗粒
胶体
生物物理学
内吞作用
化学
生物化学
酶
生物
有机化学
复合材料
水溶液
物理化学
细胞
作者
Ankan Kumar Sarkar,Koushik Debnath,Himali Arora,Pankaj Seth,Nihar Ranjan Jana,Nikhil R. Jana
标识
DOI:10.1021/acsami.1c22009
摘要
Direct cytosolic delivery of large biomolecules that bypass the endocytic pathways is a promising strategy for therapeutic applications. Recent works have shown that small-molecule, nanoparticle, and polymer-based carriers can be designed for direct cytosolic delivery. It has been shown that the specific surface chemistry of the carrier, nanoscale assembly between the carrier and cargo molecule, good colloidal stability, and low surface charge of the nano-assembly are critical for non-endocytic uptake processes. Here we report a guanidinium-terminated polyaspartic acid micelle for direct cytosolic delivery of protein and DNA. The polymer delivers the protein/DNA directly to the cytosol by forming a nano-assembly, and it is observed that <200 nm size of colloidal assembly with near-zero surface charge is critical for efficient cytosolic delivery. This work shows the importance of size and colloidal property of the nano-assembly for carrier-based cytosolic delivery of large biomolecules.
科研通智能强力驱动
Strongly Powered by AbleSci AI