重编程
生物
配子发生
体细胞
生殖系
诱导多能干细胞
外胚层
生殖系发育
细胞生物学
生殖细胞
干细胞
表观遗传学
胚胎干细胞
遗传学
胚胎
细胞
基因
胚胎发生
原肠化
作者
Mami Oikawa,Hisato Kobayashi,Makoto Sanbo,Naoaki Mizuno,K Nakayama,Tomoya Takashima,Keiko Yamauchi,Fumika Yoshida,Takuya Yamamoto,Takashi Shinohara,Hiromitsu Nakauchi,Kazuki Kurimoto,Masumi Hirabayashi,Toshihiro Kobayashi
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2022-04-08
卷期号:376 (6589): 176-179
被引量:36
标识
DOI:10.1126/science.abl4412
摘要
The in vitro generation of germ cells from pluripotent stem cells (PSCs) can have a substantial effect on future reproductive medicine and animal breeding. A decade ago, in vitro gametogenesis was established in the mouse. However, induction of primordial germ cell-like cells (PGCLCs) to produce gametes has not been achieved in any other species. Here, we demonstrate the induction of functional PGCLCs from rat PSCs. We show that epiblast-like cells in floating aggregates form rat PGCLCs. The gonadal somatic cells support maturation and epigenetic reprogramming of the PGCLCs. When rat PGCLCs are transplanted into the seminiferous tubules of germline-less rats, functional spermatids-that is, those capable of siring viable offspring-are generated. Insights from our rat model will elucidate conserved and divergent mechanisms essential for the broad applicability of in vitro gametogenesis.
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