适体
检出限
化学
罗丹明B
荧光
胶体金
罗丹明
纳米技术
色谱法
线性范围
石墨烯
分析化学(期刊)
材料科学
纳米颗粒
分子生物学
生物
生物化学
物理
光催化
量子力学
催化作用
作者
Xingyue Chen,Lulan Yang,Jiaming Tang,Wen Xu,Xiaoling Zheng,Ling‐Ling Chen,Jiaqi Li,Yong Xie,Tao Le
出处
期刊:Biosensors
[MDPI AG]
日期:2022-07-11
卷期号:12 (7): 513-513
被引量:3
摘要
Herein, we developed a novel truncation technique for aptamer sequences to fabricate highly sensitive aptasensors based on molecular docking and molecular dynamics simulations. The binding mechanism and energy composition of the aptamer/sulfaquinoxaline (SQX) complexes were investigated. We successfully obtained a new SQX-specific aptamer (SBA28-1: CCCTAGGGG) with high affinity (Kd = 27.36 nM) and high specificity determined using graphene oxide. This aptamer has a unique stem-loop structure that can bind to SQX. Then, we fabricated a fluorescence aptasensor based on SBA28-1, gold nanoparticles (AuNPs), and rhodamine B (RhoB) that presented a good linear range of 1.25-160 ng/mL and a limit of detection of 1.04 ng/mL. When used to analyze water samples, the aptasensor presented acceptable recovery rates of 93.1-100.1% and coefficients of variation (CVs) of 2.2-10.2%. In conclusion, the fluorescence aptasensor can accurately and sensitively detect SQX in water samples and has good application prospects.
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