生物正交化学
点击化学
化学
反应性(心理学)
叠氮化物
聚乙二醇
组合化学
环加成
磁性纳米粒子
膜
纳米颗粒
衍生工具(金融)
共价键
纳米技术
有机化学
生物化学
材料科学
金融经济学
病理
催化作用
经济
替代医学
医学
作者
Javier Idiago-López,Eduardo Moreno-Antolín,Maite Eceiza,J. M. Aizpurua,Valeria Grazú,Jesús M. de la Fuente,Raluca M. Fratila
标识
DOI:10.1021/acs.bioconjchem.2c00230
摘要
In this work, we report the use of bioorthogonal chemistry, specifically the strain-promoted click azide-alkyne cycloaddition (SPAAC) for the covalent attachment of magnetic nanoparticles (MNPs) on living cell membranes. Four types of MNPs were prepared, functionalized with two different stabilizing/passivation agents (a polyethylene glycol derivative and a glucopyranoside derivative, respectively) and two types of strained alkynes with different reactivities: a cyclooctyne (CO) derivative and a dibenzocyclooctyne (DBCO) derivative. The MNPs were extensively characterized in terms of physicochemical characteristics, colloidal stability, and click reactivity in suspension. Then, the reactivity of the MNPs toward azide-modified surfaces was evaluated as a closer approach to their final application in a living cell scenario. Finally, the DBCO-modified MNPs, showing superior reactivity in suspension and on surfaces, were selected for cell membrane immobilization via the SPAAC reaction on the membranes of cells engineered to express azide artificial reporters. Overall, our work provides useful insights into the appropriate surface engineering of nanoparticles to ensure a high performance in terms of bioorthogonal reactivity for biological applications.
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