清脆的
检出限
核酸扩增试验
环介导等温扩增
病毒学
分子生物学
生物
质粒
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
化学
DNA
2019年冠状病毒病(COVID-19)
遗传学
基因
色谱法
医学
病理
传染病(医学专业)
沙眼衣原体
疾病
作者
Yuanhao Liang,Hongqing Lin,Lirong Zou,Xiaoling Deng,Shixing Tang
标识
DOI:10.1016/j.bios.2022.114098
摘要
The newly emerged SARS-CoV-2 variant of concern (VOC) Omicron is spreading quickly worldwide, which manifests an urgent need of simple and rapid assay to detect and diagnose Omicron infection and track its spread. To design allele-specific CRISPR RNAs (crRNAs) targeting the signature mutations in the spike protein of Omicron variant, and to develop a CRISPR-Cas12a-based assay to specifically detect Omicron variant. Our system showed a low limit of detection of 2 copies per reaction for the plasmid DNA of Omicron variant, and could readily detect Omicron variant in 5 laboratory-confirmed clinical samples and distinguish them from 57 SARS-CoV-2 positive clinical samples (4 virus isolates and 53 oropharyngeal swab specimens) infected with wild-type (N = 8) and the variants of Alpha (N = 17), Beta (N = 17) and Delta (N = 15). The testing results could be measured by fluorescent detector or judged by naked eyes. In addition, no cross-reaction was observed when detecting 16 clinical samples infected with 9 common respiratory pathogens. The rapid assay could be easily set up in laboratories already conducting SARS-CoV-2 nucleic acid amplification tests and implemented routinely in resource-limited settings to monitor and track the spread of Omicron variant.
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